Romero-Calle Danitza Xiomara, Pedrosa-Silva Francisnei, Ribeiro Tomé Luiz Marcelo, Fonseca Vagner, Guimarães Benevides Raquel, de Oliveira Santos Leila Thaise Santana, de Oliveira Tulio, da Costa Mateus Matiuzzi, Alcantara Luiz Carlos Junior, de Carvalho Azevedo Vasco Ariston, Brenig Bertram, Venancio Thiago M, Billington Craig, Góes-Neto Aristóteles
Postgraduate Program in Biotechnology, State University of Feira de Santana (UEFS), Av. Transnordestina S/N, Feira de Santana 44036-900, Brazil.
Molecular and Computational Biology of Fungi Laboratory, Department of Microbiology, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Brazil.
Microorganisms. 2023 Jul 19;11(7):1837. doi: 10.3390/microorganisms11071837.
Antimicrobial resistance is increasing despite new treatments being employed, so novel strategies are required to ensure that bacterial infections remain treatable. Bacteriophages (phages; bacteria viruses) have the potential to be used as natural antimicrobial methods to control bacterial pathogens such as spp. A phage, Wara, was isolated from environmental water samples at the Subaé River Basin, Salvador de Bahia, Brazil. The basin has environmental impacts in its main watercourses arising from the dumping of domestic and industrial effluents and agricultural and anthropological activities. The phage genome sequence was determined by Oxford Nanopore Technologies (ONT) MinION and Illumina HiSeq sequencing, and assembly was carried out by Racon (MinION) and Unicycler (Illumina, Illumina + MinION). The genome was annotated and compared to other phages using various bioinformatics approaches. MinION DNA sequencing combined with Racon assembly gave the best complete genome sequence. Phylogenetic analysis revealed that Wara is a member of the genus. A lack of lysogeny genes, antimicrobial resistance, and virulence genes indicated that Wara has therapeutic and biocontrol potential against species in healthcare and agriculture.
尽管采用了新的治疗方法，但抗菌药物耐药性仍在增加，因此需要新的策略来确保细菌感染仍然可以治疗。噬菌体（细菌病毒）有潜力被用作控制诸如 spp. 等细菌病原体的天然抗菌方法。一种名为瓦拉（Wara）的噬菌体是从巴西巴伊亚州萨尔瓦多苏巴埃河流域的环境水样中分离出来的。该流域的主要水道受到生活和工业废水排放以及农业和人类活动的环境影响。噬菌体基因组序列通过牛津纳米孔技术公司（ONT）的MinION和Illumina HiSeq测序确定，并通过Racon（MinION）和Unicycler（Illumina、Illumina + MinION）进行组装。使用各种生物信息学方法对基因组进行注释并与其他噬菌体进行比较。MinION DNA测序与Racon组装相结合得到了最佳的完整基因组序列。系统发育分析表明瓦拉是 属的成员。缺乏溶原性基因、抗菌耐药性和毒力基因表明瓦拉在医疗保健和农业中对 物种具有治疗和生物防治潜力。
