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分析一种新型噬菌体 vB_AchrS_AchV4 突出了病毒的多样性。

Analysis of a Novel Bacteriophage vB_AchrS_AchV4 Highlights the Diversity of Viruses.

机构信息

Department of Molecular Microbiology and Biotechnology, Institute of Biochemistry, Life Sciences Center, Vilnius University, Saulėtekio av. 7, LT-10257 Vilnius, Lithuania.

Proteomics Centre, Institute of Biochemistry, Life Sciences Centre, Vilnius University, Saulėtekio av. 7, LT-10257 Vilnius, Lithuania.

出版信息

Viruses. 2021 Feb 27;13(3):374. doi: 10.3390/v13030374.

DOI:10.3390/v13030374
PMID:33673419
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7996906/
Abstract

spp. are ubiquitous in nature and are increasingly being recognized as emerging nosocomial pathogens. Nevertheless, to date, only 30 complete genome sequences of phages are available in GenBank, and nearly all of those phages were isolated on . Here, we report the isolation and characterization of bacteriophage vB_AchrS_AchV4. To the best of our knowledge, vB_AchrS_AchV4 is the first virus isolated from . Both vB_AchrS_AchV4 and its host, RL_4, were isolated in Lithuania. VB_AchrS_AchV4 is a siphovirus, since it has an isometric head (64 ± 3.2 nm in diameter) and a non-contractile flexible tail (232 ± 5.4). The genome of vB_AchrS_AchV4 is a linear dsDNA molecule of 59,489 bp with a G+C content of 62.8%. It contains no tRNA genes, yet it includes 82 protein-coding genes, of which 27 have no homologues in phages. Using bioinformatics approaches, 36 vB_AchrS_AchV4 genes were given a putative function. A further four were annotated based on the results of LC-MS/MS. Comparative analyses revealed that vB_AchrS_AchV4 is a singleton siphovirus with no close relatives among known tailed phages. In summary, this work not only describes a novel and unique phage, but also advances our knowledge of genetic diversity and evolution of bacteriophages.

摘要

spp. 在自然界中无处不在，并且越来越被认为是新兴的医院病原体。尽管如此，迄今为止，GenBank 中仅提供了 30 个完整的噬菌体基因组序列，而且几乎所有这些噬菌体都是在. 上分离得到的。在这里，我们报告了噬菌体 vB_AchrS_AchV4 的分离和特性。据我们所知，vB_AchrS_AchV4 是从. 中分离出来的第一种病毒。vB_AchrS_AchV4 和它的宿主 RL_4 都是在立陶宛分离得到的。vB_AchrS_AchV4 是一种肌尾噬菌体，因为它具有等轴头（直径为 64 ± 3.2nm）和非收缩性柔性尾巴（232 ± 5.4）。vB_AchrS_AchV4 的基因组是一个线性 dsDNA 分子，长 59489bp，GC 含量为 62.8%。它不含 tRNA 基因，但包含 82 个编码蛋白质的基因，其中 27 个在噬菌体中没有同源物。使用生物信息学方法，赋予了 36 个 vB_AchrS_AchV4 基因一个假定的功能。另外四个基因是根据 LC-MS/MS 的结果进行注释的。比较分析表明，vB_AchrS_AchV4 是一种独特的肌尾噬菌体，在已知的有尾噬菌体中没有密切相关的亲缘关系。总之，这项工作不仅描述了一种新型独特的噬菌体，还增进了我们对噬菌体遗传多样性和进化的了解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d67a/7996906/413979bac3cb/viruses-13-00374-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d67a/7996906/538b57d42146/viruses-13-00374-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d67a/7996906/5a8767bbb993/viruses-13-00374-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d67a/7996906/b02bc0b1375f/viruses-13-00374-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d67a/7996906/8f724743e94f/viruses-13-00374-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d67a/7996906/5da762c58ef2/viruses-13-00374-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d67a/7996906/0bbf5b458b92/viruses-13-00374-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d67a/7996906/413979bac3cb/viruses-13-00374-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d67a/7996906/538b57d42146/viruses-13-00374-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d67a/7996906/5a8767bbb993/viruses-13-00374-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d67a/7996906/b02bc0b1375f/viruses-13-00374-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d67a/7996906/8f724743e94f/viruses-13-00374-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d67a/7996906/5da762c58ef2/viruses-13-00374-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d67a/7996906/0bbf5b458b92/viruses-13-00374-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d67a/7996906/413979bac3cb/viruses-13-00374-g007.jpg

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