改良的恶性疟原虫环状体生存检测法及 ML10 激酶抑制剂的应用
Modified Plasmodium falciparum Ring-Stage Survival Assay with ML10 Kinase Inhibitor.
机构信息
Institut Pasteur, Université Paris Cité, Malaria Genetics and Resistance Unit, INSERM U1201, Paris, France.
Sorbonne Université, Paris, France.
出版信息
Antimicrob Agents Chemother. 2023 May 17;67(5):e0001723. doi: 10.1128/aac.00017-23. Epub 2023 Apr 26.
Abstract
The ring-stage survival assay is the reference assay to measure Plasmodium falciparum artemisinin partial resistance. The main challenge of the standard protocol is to generate 0-to-3-h postinvasion ring stages (the stage least susceptible to artemisinin) from schizonts obtained by sorbitol treatment and Percoll gradient. We report here a modified protocol facilitating the production of synchronized schizonts when multiple strains are tested simultaneously, by using ML10, a protein kinase inhibitor, that reversibly blocks merozoite egress.
摘要
环体生存检测是测量恶性疟原虫青蒿素部分耐药性的参考检测方法。标准方案的主要挑战是从经山梨醇处理和 Percoll 梯度获得的裂殖体中产生 0 至 3 小时的入侵环体阶段（对青蒿素最不敏感的阶段）。我们在此报告一种改良方案，该方案通过使用可逆性阻断裂殖体逸出的蛋白激酶抑制剂 ML10，可在同时检测多个菌株时促进同步化裂殖体的产生。
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