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采用指甲花提取液合成的银纳米粒子对尿路感染常见病原体的抗菌活性。

Antibacterial Activity of Green Synthesized Silver Nanoparticles Using Lawsonia inermis Against Common Pathogens from Urinary Tract Infection.

机构信息

Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Nasr City, 11884, Cairo, Egypt.

出版信息

Appl Biochem Biotechnol. 2024 Jan;196(1):85-98. doi: 10.1007/s12010-023-04482-1. Epub 2023 Apr 26.

DOI:10.1007/s12010-023-04482-1
PMID:37099124
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10794286/
Abstract

New and creative methodologies for the fabrication of silver nanoparticles (Ag-NPs), which are exploited in a wide range of consumer items, are of significant interest. Hence, this research emphasizes the biological approach of Ag-NPs through Egyptian henna leaves (Lawsonia inermis Linn.) extracts and analysis of the prepared Ag-NPs. Plant extract components were identified by gas chromatography mass spectrometry (GC-mass). The analyses of prepared Ag-NPs were carried out through UV-visible (UV-Vis), X-ray diffraction (XRD), transmission electron microscope (TEM), scanning electron microscope (SEM), and Fourier transform infrared (FTIR) analysis. UV-Vis reveals that Ag-NPs have a maximum peak at 460 nm in visible light. Structural characterization recorded peaks that corresponded to Bragg's diffractions for silver nano-crystal, with average crystallite sizes varying from 28 to 60 nm. Antibacterial activities of Ag-NPs were examined, and it is observed that all microorganisms are very sensitive to biologically synthesized Ag-NPs.

摘要

新型和创新的银纳米粒子（Ag-NPs）制造方法在广泛的消费品中得到了应用，这引起了人们的极大兴趣。因此，本研究强调了通过埃及指甲花（Lawsonia inermis Linn.）提取物和制备的 Ag-NPs 分析来实现 Ag-NPs 的生物方法。通过气相色谱质谱联用仪（GC-MS）鉴定植物提取物成分。通过紫外可见分光光度计（UV-Vis）、X 射线衍射（XRD）、透射电子显微镜（TEM）、扫描电子显微镜（SEM）和傅里叶变换红外光谱（FTIR）分析对制备的 Ag-NPs 进行分析。UV-Vis 显示 Ag-NPs 在可见光下的最大峰值为 460nm。结构特征记录的峰与银纳米晶体的布拉格衍射相对应，平均晶粒尺寸在 28 至 60nm 之间变化。Ag-NPs 的抗菌活性也进行了检测，结果表明所有微生物对生物合成的 Ag-NPs 都非常敏感。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b048/10794286/10b2f808066d/12010_2023_4482_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b048/10794286/953e147fdc3c/12010_2023_4482_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b048/10794286/91f1f8afb603/12010_2023_4482_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b048/10794286/eba52cbbe518/12010_2023_4482_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b048/10794286/6fac9ba71978/12010_2023_4482_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b048/10794286/10b2f808066d/12010_2023_4482_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b048/10794286/953e147fdc3c/12010_2023_4482_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b048/10794286/91f1f8afb603/12010_2023_4482_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b048/10794286/eba52cbbe518/12010_2023_4482_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b048/10794286/6fac9ba71978/12010_2023_4482_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b048/10794286/10b2f808066d/12010_2023_4482_Fig5_HTML.jpg

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