Rede Nordeste de Biotecnologia, Universidade Federal do Ceará, Campus do Pici, Fortaleza, CE CEP 60455-760, Brazil.
Departamento de Engenharia Química, Universidade Federal do Ceará, Campus do Pici, bloco 709, Fortaleza, CE CEP 60455-760, Brazil.
Int J Biol Macromol. 2023 Jul 1;242(Pt 1):124405. doi: 10.1016/j.ijbiomac.2023.124405. Epub 2023 Apr 25.
The industrial residue of cashew apple juice processing (MRC) was evaluated as an alternative medium for bacterial cellulose (BC) production by Komagataeibacter xylinus ATCC 53582 and Komagataeibacter xylinus ARS B42. The synthetic Hestrin-Schramm medium (MHS) was used as a control for growing and BC production. First, BC production was assessed after 4, 6, 8, 10, and 12 days under static culture. After 12 days of cultivation, K. xylinus ATCC 53582 produced the highest BC titer in MHS (3.1 g·L) and MRC (3 g·L), while significant productivity was attained at 6 days of fermentation. To understand the effect of culture medium and fermentation time on the properties of the obtained films, BC produced at 4, 6, or 8 days were submitted to infrared spectroscopy with Fourier transform, thermogravimetry, mechanical tests, water absorption capacity, scanning electron microscopy, degree of polymerization and X-ray diffraction. The properties of BC synthesized in MRC were identical to those of BC from MHS, according to structural, physical, and thermal studies. MRC, on the other hand, allows the production of BC with a high water absorption capacity when compared to MHS. Despite the lower titer (0.88 g·L) achieved in MRC, the BC from K. xylinus ARS B42 presented a high thermal resistance and a remarkable absorption capacity (14664 %), suggesting that it might be used as a superabsorbent biomaterial.
腰果苹果汁加工的工业残渣（MRC）被评估为可替代的细菌纤维素（BC）生产介质，用于木醋杆菌 ATCC 53582 和木醋杆菌 ARS B42。合成的 Hestrin-Schramm 培养基（MHS）被用作生长和 BC 生产的对照。首先，在静态培养下评估 4、6、8、10 和 12 天后的 BC 生产情况。经过 12 天的培养，K. xylinus ATCC 53582 在 MHS（3.1 g·L）和 MRC（3 g·L）中产生了最高的 BC 产量，而在发酵 6 天时达到了显著的生产力。为了了解培养基和发酵时间对所得薄膜性能的影响，将在 4、6 或 8 天发酵生产的 BC 进行傅里叶变换红外光谱、热重分析、机械测试、吸水率、扫描电子显微镜、聚合度和 X 射线衍射分析。根据结构、物理和热研究，在 MRC 中合成的 BC 的性能与在 MHS 中合成的 BC 相同。另一方面，与 MHS 相比，MRC 允许生产具有高吸水率的 BC。尽管在 MRC 中实现的产量较低（0.88 g·L），但 K. xylinus ARS B42 的 BC 表现出较高的热稳定性和显著的吸水率（14664%），表明其可用作超吸水性生物材料。
