基于STAT1/3信号通路的清解退热颗粒抗流感机制的网络药理学研究
Network pharmacology associated anti-influenza mechanism research of Qingjie-Tuire Granule via STAT1/3 signaling pathway.
作者信息
Wang Yutao, Zhao Xin, Xiao Mengjie, Lin Xiaoying, Chen Qiaolian, Qin Shengle, Ti Huihui, Yang Zifeng
机构信息
State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510180, China.
Guangdong Provincial Key Laboratory of Chemical Measurement and Emergency Test Technology, Institute of Analysis, Guangdong Academy of Sciences (China National Analytical Center, Guangzhou), Guangzhou, 510070, China.
出版信息
Heliyon. 2023 Mar 20;9(3):e14649. doi: 10.1016/j.heliyon.2023.e14649. eCollection 2023 Mar.
Qingjie-Tuire (QT) granule was approved for clinical use and its combination was reported to treat influenza infection. To explore its active component and mechanism, the components of QT granule were retrieved from UPLC-UC-Q-TOF/MS analysis. The genes corresponding to the targets were retrieved using GeneCards and TTD database. The herb-compound-target network was constructed by Cytoscape. The target protein-protein interaction network was built using STRING database. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses of QT granule to IAV were performed for further study. The regulation to different signaling transduction events and cytokine/chemokine expression of QT granule was evaluated using Western blotting and real-time qPCR. Totally, 47 compounds were identified and effect of QT granule on cell STAT1/3 signaling pathways was confirmed by A549 cell model. The efficiency of QT granule on host cell contributes to its clinical application and mechanism research.
清解退热（QT）颗粒已获批用于临床，据报道其复方可治疗流感感染。为探究其活性成分及作用机制，通过超高效液相色谱-高分辨质谱联用（UPLC-UC-Q-TOF/MS）分析检索QT颗粒的成分。利用GeneCards和TTD数据库检索与靶点对应的基因。通过Cytoscape构建草药-化合物-靶点网络。使用STRING数据库构建靶点蛋白-蛋白相互作用网络。对QT颗粒针对甲型流感病毒（IAV）进行基因本体（GO）和京都基因与基因组百科全书（KEGG）富集分析以作进一步研究。采用蛋白质免疫印迹法和实时定量聚合酶链反应评估QT颗粒对不同信号转导事件及细胞因子/趋化因子表达的调控作用。共鉴定出47种化合物，并通过A549细胞模型证实了QT颗粒对细胞信号转导和转录激活因子1/3（STAT1/3）信号通路的影响。QT颗粒对宿主细胞的作用效率有助于其临床应用及作用机制研究。
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