采用新型扫描四极杆数据非依赖型采集策略，通过液相色谱/质谱联用对复杂生物混合物进行脂质分析。
Lipid profiling of complex biological mixtures by liquid chromatography/mass spectrometry using a novel scanning quadrupole data-independent acquisition strategy.
作者信息
Gethings Lee A, Richardson Keith, Wildgoose Jason, Lennon Sarah, Jarvis Sheba, Bevan Charlotte L, Vissers Johannes P C, Langridge James I
机构信息
Waters Corporation, Stamford Avenue, Wilmslow, UK.
Department of Surgery and Cancer, Imperial College, London, UK.
出版信息
Rapid Commun Mass Spectrom. 2017 Oct 15;31(19):1599-1606. doi: 10.1002/rcm.7941.
RATIONALE
A novel data-independent acquisition method is detailed that incorporates a scanning quadrupole in front of an orthogonal acceleration time-of-flight (TOF) mass analyser. This approach is described and the attributes are compared and contrasted to other DIA approaches.
METHODS
Specific application of the method to both targeted and untargeted lipidomic identification strategies is discussed, with data from both shotgun and LC separated lipidomics experiments presented.
RESULTS
The benefits of the fast quadrupole scanning technique are highlighted, and include improvements in speed and specificity for complex mixtures providing high quality qualitative and quantitative data.
CONCLUSIONS
In particular the high specificity afforded by the scanning quadrupole improves qualitative information for lipid identification.
原理
详细介绍了一种新型的数据非依赖型采集方法，该方法在正交加速飞行时间（TOF）质量分析器前集成了一个扫描四极杆。描述了这种方法，并将其特性与其他数据非依赖型采集方法进行了比较和对比。
方法
讨论了该方法在靶向和非靶向脂质组学鉴定策略中的具体应用，并展示了来自鸟枪法和液相色谱分离脂质组学实验的数据。
结果
强调了快速四极杆扫描技术的优势，包括提高了复杂混合物的分析速度和特异性，从而提供高质量的定性和定量数据。
结论
特别是扫描四极杆提供的高特异性提高了脂质鉴定的定性信息。
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