Department of Large Animal Surgery, Anaesthesia and Orthopaedics, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, Merelbeke 9820, Belgium.
Department of Large Animal Surgery, Anaesthesia and Orthopaedics, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, Merelbeke 9820, Belgium.
Res Vet Sci. 2023 Jun;159:101-105. doi: 10.1016/j.rvsc.2023.04.014. Epub 2023 Apr 23.
Bovine papillomavirus (BPV) types 1 and 2 are causally associated with equine sarcoid, the most common mesenchymal neoplasm of horses, but the viral load (VL) differs between lesions. Sensitive and accurate BPV detection and quantification is essential for clinicians to confirm clinical suspicion, as well as in research settings for stratifying these skin lesions. Due to the limitations of histopathology in sarcoid diagnosis, PCR screening of superficial swabs constitutes the principal sampling method for BPV detection. This study aimed to investigate the ability of superficial swabs and fine-needle aspirates (FNA) to accurately detect the VL in equine sarcoids, considering the main clinical types: occult, nodular, verrucous and fibroblastic. Superficial swabs and FNAs from a series of sarcoid-affected horses were tested in parallel for BPV DNA quantification. Quantitative real-time PCR screening of postoperative tissue biopsies served as reference standard for the accuracy assessment of the viral titters. Our results indicate that VL is not a predictor of the clinical type. Student's t-test results gave evidence of a significant difference between both sample methods (P < 0.001) with FNA giving the best approximation of the actual VL (P < 0.01). In contrast to superficial swabs, the reference standard correlated moderately with FNA in general (P < 0.05; r = 0.39) and strongly with FNA results within the occult sarcoid group (P < 0.05; r = 0.59). In conclusion, the correlation of FNA with the reference standard was strong enough to suggest this is the preferred method for quantifying VL in sarcoids.
牛乳头瘤病毒（BPV）1 型和 2 型与马的最常见间叶性肿瘤——结节性皮肤病有因果关系，但病变之间的病毒载量（VL）不同。临床医生需要对 BPV 进行敏感和准确的检测和定量，以确认临床怀疑，同时在研究中对这些皮肤病变进行分层。由于组织病理学在结节性皮肤病诊断中的局限性，表面拭子的 PCR 筛查构成了 BPV 检测的主要采样方法。本研究旨在探讨表面拭子和细针抽吸（FNA）在准确检测马结节性皮肤病 VL 方面的能力，考虑到主要的临床类型：隐匿型、结节型、疣状型和纤维母细胞型。对一系列结节性皮肤病马的表面拭子和 FNA 进行了平行检测，以定量 BPV DNA。术后组织活检的定量实时 PCR 筛查被用作病毒滴度准确性评估的参考标准。我们的研究结果表明，VL 不是临床类型的预测因素。学生 t 检验结果表明，两种样本方法之间存在显著差异（P < 0.001），FNA 能更好地近似实际 VL（P < 0.01）。与表面拭子不同，参考标准与 FNA 一般相关性中等（P < 0.05；r = 0.39），与隐匿性结节性皮肤病组的 FNA 结果相关性较强（P < 0.05；r = 0.59）。总之，FNA 与参考标准的相关性足够强，表明这是定量结节性皮肤病 VL 的首选方法。
