de Andrade Iara Bastos, Corrêa-Junior Dario, Alves Vinicius, Figueiredo-Carvalho Maria Helena Galdino, Santos Marcos Vinicius, Almeida Marcos Abreu, Valdez Alessandro Fernandes, Nimrichter Leonardo, Almeida-Paes Rodrigo, Frases Susana
Laboratório de Biofísica de Fungos, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-902, Brazil.
Laboratório de Micologia, Instituto Nacional de Infectologia Evandro Chagas, Fundação Oswaldo Cruz, Rio de Janeiro 21941-902, Brazil.
J Fungi (Basel). 2023 Apr 18;9(4):487. doi: 10.3390/jof9040487.
This study aimed to investigate the effects of cyclosporine on the morphology, cell wall structure, and secretion characteristics of . The minimum inhibitory concentration (MIC) of cyclosporine was found to be 2 µM (2.4 µg/mL) for the H99 strain. Yeast cells treated with cyclosporine at half the MIC showed altered morphology, including irregular shapes and elongated projections, without an effect on cell metabolism. Cyclosporine treatment resulted in an 18-fold increase in chitin and an 8-fold increase in lipid bodies, demonstrating changes in the fungal cell wall structure. Cyclosporine also reduced cell body and polysaccharide capsule diameters, with a significant reduction in urease secretion in cultures. Additionally, the study showed that cyclosporine increased the viscosity of secreted polysaccharides and reduced the electronegativity and conductance of cells. The findings suggest that cyclosporine has significant effects on morphology, cell wall structure, and secretion, which could have implications for the development of new antifungal agents.
本研究旨在探讨环孢素对……的形态、细胞壁结构和分泌特性的影响。发现环孢素对H99菌株的最低抑菌浓度（MIC）为2 µM（2.4 µg/mL）。用半MIC的环孢素处理酵母细胞后，细胞形态发生改变，包括形状不规则和出现细长突起，但对细胞代谢无影响。环孢素处理导致几丁质增加18倍，脂质体增加8倍，表明真菌细胞壁结构发生了变化。环孢素还减小了细胞体和多糖荚膜的直径，在……培养物中脲酶分泌显著减少。此外，研究表明环孢素增加了分泌多糖的粘度，并降低了细胞的负电性和电导率。这些发现表明，环孢素对……的形态、细胞壁结构和分泌有显著影响，这可能对新型抗真菌药物的开发具有重要意义。
