丙二醛（MDA）在慢性脊髓损伤时间进程中的预后价值
Prognostic Value of Malondialdehyde (MDA) in the Temporal Progression of Chronic Spinal Cord Injury.
作者信息
Haro Girón Sergio, Monserrat Sanz Jorge, Ortega Miguel A, Garcia-Montero Cielo, Fraile-Martínez Oscar, Gómez-Lahoz Ana M, Boaru Diego Liviu, de Leon-Oliva Diego, Guijarro Luis G, Atienza-Perez Mar, Diaz David, Lopez-Dolado Elisa, Álvarez-Mon Melchor
机构信息
Department of Medicine and Medical Specialities, Faculty of Medicine and Health Sciences, University of Alcalá, 28801 Alcalá de Henares, Spain.
Ramón y Cajal Institute of Sanitary Research (IRYCIS), 28034 Madrid, Spain.
出版信息
J Pers Med. 2023 Apr 2;13(4):626. doi: 10.3390/jpm13040626.
BACKGROUND
Oxidative stress is a major signature of spinal cord injury (SCI). The altered levels of various oxidative stress markers have been demonstrated in acute and chronic SCI. However, the variation of these markers in patients with chronic SCI depending on the time since the initial injury has not been explored yet.
OBJECTIVE
Our aim was to measure plasma levels of malondialdehyde (MDA), a marker of lipid peroxidation in patients with SCI stratified in different periods of suffering the injury (0-5 years, 5-10 years, and more than 10 years).
PATIENTS AND METHODS
This cross-sectional study enrolled patients with SCI (N = 105) from different periods of the lesion and healthy control (HC) subjects (N = 38): short period (SCI SP, N = 31, time of evolution less than 5 years); early chronic (SCI ECP, N = 32, time of evolution 5-15 years); and late chronic (SCI LCP, N = 42, time of evolution more than 15 years). The plasma levels of MDA were measured using a commercially available colorimetric assay.
RESULTS
Patients with SCI had significantly higher plasma levels of MDA than HC subjects. Receiver operating characteristic (ROC) curve analysis for plasma MDA levels in patients with SCI demonstrated areas under the curve (AUC) of 1 (HC vs. SCI-SP); 0.998 (HC vs. SCI-ECP); and 0.964 (HC vs. SCI-LCP). Additionally, three ROC curves were used to compare the different concentrations of MDA between the subgroups of patients with SCI, and the resulting AUCs were: 0.896 (SCI-SP vs. SCI-ECP); 0.840 (SCI-ECP vs. SCI-LCP); and 0.979 (SCI-SP vs. SCI-LCP).
CONCLUSION
Plasma concentration of MDA can be considered as an oxidative stress biomarker to assess the prognosis of SCI in chronic stages.
背景
氧化应激是脊髓损伤（SCI）的主要特征。在急性和慢性脊髓损伤中，各种氧化应激标志物的水平均发生了改变。然而，慢性脊髓损伤患者中这些标志物随初次损伤后时间的变化情况尚未得到研究。
目的
我们的目的是测量脊髓损伤患者在不同损伤时期（0 - 5年、5 - 10年和10年以上）分层后血浆中丙二醛（MDA）的水平，丙二醛是脂质过氧化的标志物。
患者与方法
这项横断面研究纳入了来自不同损伤时期的脊髓损伤患者（N = 105）和健康对照（HC）受试者（N = 38）：短期（脊髓损伤短期组，SCI SP，N = 31，病程小于5年）；早期慢性期（脊髓损伤早期慢性组，SCI ECP，N = 32，病程5 - 15年）；晚期慢性期（脊髓损伤晚期慢性组，SCI LCP，N = 42，病程超过15年）。使用市售的比色测定法测量血浆中MDA的水平。
结果
脊髓损伤患者的血浆MDA水平显著高于健康对照受试者。对脊髓损伤患者血浆MDA水平进行的受试者工作特征（ROC）曲线分析显示，曲线下面积（AUC）分别为：1（HC与SCI - SP比较）；0.998（HC与SCI - ECP比较）；以及0.964（HC与SCI - LCP比较）。此外，使用三条ROC曲线比较脊髓损伤患者亚组之间不同浓度的MDA，所得AUC分别为：0.896（SCI - SP与SCI - ECP比较）；0.840（SCI - ECP与SCI - LCP比较）；以及0.979（SCI - SP与SCI - LCP比较）。
结论
血浆MDA浓度可被视为评估慢性期脊髓损伤预后的氧化应激生物标志物。
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