Prevalence of Oral Pathogen among Clinical Orthodontic and Non-Orthodontic Saliva Samples.

(SE) is a recently identified intestinal microbe, which recent oral surveys suggest may be associated with oral diseases including caries and periodontal disease. Based upon the lack of information regarding this organism, the primary objective of this study was to determine the oral prevalence of this microbe and any potential associations with patient characteristics such as age, sex, or the presence of orthodontic appliances. This retrospective study involved the screening of an existing saliva repository composed of previously collected unstimulated clinical saliva samples. More specifically, N = 266 were identified and screened using a spectrophotometer at absorbances of A260 and A280 nm to determine their DNA purity and concentration. qPCR screening of these samples revealed a higher prevalence of positive samples among pediatric patients (63.1%) compared with adults (36.9%) in this clinic population, = 0.0007. In addition, higher percentages of were observed among orthodontic patients (71.2%) compared with non-orthodontic patients (28.8%), = 0.0001. These results did not vary by sex with nearly equal percentages of positive males and females among adult and pediatric patients, as well as orthodontic and non-orthodontic samples. These results suggest a strong potential for association between the prevalence of this organism with age as well as orthodontic status, given that younger patients and those with orthodontic brackets (regardless of age) were most likely to harbor this pathogen in sufficient levels to be detected in saliva. More research will be needed to determine any associations with specific outcomes, such as caries or periodontal disease, among positive patients within these specific populations.