Shen Ching, Simpson Justin, Clawson James Brigham, Lam Steven, Kingsley Karl
Department of Advanced Education in Orthodontic Dentistry, School of Dental Medicine, University of Nevada-Las Vegas, 1700 W. Charleston Boulevard, Las Vegas, NV 89106, USA.
Department of Clinical Sciences, School of Dental Medicine, University of Nevada-Las Vegas, 1700 W. Charleston Boulevard, Las Vegas, NV 89106, USA.
Microorganisms. 2023 Mar 28;11(4):867. doi: 10.3390/microorganisms11040867.
(SE) is a recently identified intestinal microbe, which recent oral surveys suggest may be associated with oral diseases including caries and periodontal disease. Based upon the lack of information regarding this organism, the primary objective of this study was to determine the oral prevalence of this microbe and any potential associations with patient characteristics such as age, sex, or the presence of orthodontic appliances. This retrospective study involved the screening of an existing saliva repository composed of previously collected unstimulated clinical saliva samples. More specifically, N = 266 were identified and screened using a spectrophotometer at absorbances of A260 and A280 nm to determine their DNA purity and concentration. qPCR screening of these samples revealed a higher prevalence of positive samples among pediatric patients (63.1%) compared with adults (36.9%) in this clinic population, = 0.0007. In addition, higher percentages of were observed among orthodontic patients (71.2%) compared with non-orthodontic patients (28.8%), = 0.0001. These results did not vary by sex with nearly equal percentages of positive males and females among adult and pediatric patients, as well as orthodontic and non-orthodontic samples. These results suggest a strong potential for association between the prevalence of this organism with age as well as orthodontic status, given that younger patients and those with orthodontic brackets (regardless of age) were most likely to harbor this pathogen in sufficient levels to be detected in saliva. More research will be needed to determine any associations with specific outcomes, such as caries or periodontal disease, among positive patients within these specific populations.
（SE）是一种最近才被发现的肠道微生物，近期的口腔调查表明它可能与包括龋齿和牙周病在内的口腔疾病有关。鉴于关于这种微生物的信息匮乏，本研究的主要目的是确定这种微生物在口腔中的流行情况以及与患者特征（如年龄、性别或正畸矫治器的使用情况）之间的任何潜在关联。这项回顾性研究涉及对一个现有的唾液库进行筛查，该唾液库由先前收集的未刺激临床唾液样本组成。更具体地说，共识别出N = 266个样本，并使用分光光度计在260和280纳米吸光度下进行筛查，以确定其DNA纯度和浓度。对这些样本进行的qPCR筛查显示，在该诊所人群中，儿科患者的阳性样本患病率（63.1%）高于成人（36.9%），P = 0.0007。此外，正畸患者中的阳性样本百分比（71.2%）高于非正畸患者（28.8%），P = 0.0001。这些结果在性别上没有差异，在成人和儿科患者以及正畸和非正畸样本中，阳性男性和女性的百分比几乎相等。这些结果表明，鉴于年轻患者和佩戴正畸托槽的患者（无论年龄）最有可能携带这种病原体，且其水平足以在唾液中被检测到，这种微生物的流行率与年龄以及正畸状态之间存在很强的潜在关联。需要更多的研究来确定在这些特定人群中，SE阳性患者与龋齿或牙周病等特定结果之间的任何关联。
