Shen Ching, Clawson James Brigham, Simpson Justin, Kingsley Karl
Department of Advanced Education in Orthodontic Dentistry, School of Dental Medicine, University of Nevada-Las Vegas, 1700 W. Charleston Boulevard, Las Vegas, NV 89106, USA.
Department of Clinical Sciences, School of Dental Medicine, University of Nevada-Las Vegas, 1700 W. Charleston Boulevard, Las Vegas, NV 89106, USA.
Microorganisms. 2023 Jan 1;11(1):112. doi: 10.3390/microorganisms11010112.
(AM) is one of many highly abundant intestinal microbes that influences homeostasis and metabolic disorders and may also play a role in oral disorders. However, there is little evidence regarding the oral prevalence of this organism. Based upon this lack of evidence, the primary goal of this project is to survey an existing saliva repository to determine the overall prevalence of this organism and any associations with demographic or patient characteristics (age, sex, body mass index, race/ethnicity, orthodontic therapy). Using an approved protocol,, a total = 141 pediatric samples from an existing saliva repository were screened using qPCR revealing 29.8% harbored AM with nearly equal distribution among males and females, = 0.8347. Significantly higher percentages of pediatric, non-orthodontic patients were positive for AM (42.3%) compared with age-matched orthodontic patients (14.3%)-which were equally distributed among non-orthodontic males (42.1%) and non-orthodontic females (42.5%). In addition, analysis of the adult samples revealed that nearly equal percentages of males (18.2%) and females (16.7%) harbored detectable levels of salivary AM, = 0.2035. However, a higher proportion of non-orthodontic adult samples harbored AM (21.3%) compared to orthodontic samples (12.8%, = 0.0001), which was equally distributed among males and females. These results suggest that both age and the presence of orthodontic brackets may influence microbial composition and, more specifically, are associated with reduction in AM among both pediatric and adult populations from their baseline levels.
放线菌属（AM）是众多高度丰富的肠道微生物之一，它影响体内平衡和代谢紊乱，也可能在口腔疾病中起作用。然而，关于这种微生物在口腔中的流行情况，几乎没有证据。基于这种证据的缺乏，本项目的主要目标是调查现有的唾液库，以确定这种微生物的总体流行率以及与人口统计学或患者特征（年龄、性别、体重指数、种族/民族、正畸治疗）的任何关联。使用批准的方案，对来自现有唾液库的总共141份儿科样本进行qPCR筛查，结果显示29.8%的样本中含有AM，男性和女性的分布几乎相等，P = 0.8347。与年龄匹配的正畸患者（14.3%）相比，儿科非正畸患者中AM阳性的比例显著更高（42.3%），在非正畸男性（42.1%）和非正畸女性（42.5%）中分布均匀。此外，对成人样本的分析显示，男性（18.2%）和女性（16.7%）中含有可检测水平唾液AM的比例几乎相等，P = 0.2035。然而，与正畸样本（12.8%，P = 0.0001）相比，非正畸成人样本中含有AM的比例更高（21.3%），在男性和女性中分布均匀。这些结果表明，年龄和正畸托槽的存在都可能影响微生物组成，更具体地说，与儿科和成人人群中AM从基线水平下降有关。
