Weihe Thomas, Yao Yijiao, Opitz Nevin, Wagner Robert, Krall Johanna, Schnabel Uta, Below Harald, Ehlbeck Jörg
Department of Plasma Biotechnology, Leibniz Institute for Plasma Science and Technology, 17489 Greifswald, Germany.
Department of Food & Nutritional Sciences, University of Reading, Whiteknights, Reading RG6 6AD, UK.
Microorganisms. 2023 Apr 3;11(4):932. doi: 10.3390/microorganisms11040932.
Plasma-treated water (PTW) possess anti-microbial potential against , which is observable for both suspended cells and cells organized in biofilms. Against that background, the chemical composition of PTW tends to focus. Various analytical techniques have been applied for analyses, which reveal various traceable reactive oxygen and nitrogen compounds (RONS). Based on these findings, it is our aim to generate a PTW analog (anPTW), which has been compared in its anti-microbial efficiency with freshly generated PTW. Additionally, a solution of every traceable compound of PTW has been mixed according to their PTW concentration. As references, we treated suspended cells and mature biofilms of with PTW that originates from a microwave-driven plasma source. The anti-microbial efficiency of all solutions has been tested based on a combination of a proliferation, an XTT, and a live-dead assay. The outcomes of the test proved an anti-microbial power of PTW that suggests more active ingredients than the traceable compounds HNO, HNO, and HO or the combined mixture of the analog.
等离子体处理水（PTW）对[具体对象未提及]具有抗菌潜力，这在悬浮细胞和生物膜中组织的细胞中均有体现。在此背景下，PTW的化学成分受到关注。已应用各种分析技术进行分析，揭示了各种可追踪的活性氧和氮化合物（RONS）。基于这些发现，我们的目标是生成一种PTW类似物（anPTW），并将其抗菌效率与新生成的PTW进行比较。此外，根据PTW中每种可追踪化合物的浓度将其溶液混合。作为对照，我们用源自微波驱动等离子体源的PTW处理[具体对象未提及]的悬浮细胞和成熟生物膜。基于增殖、XTT和活死检测的组合测试了所有溶液的抗菌效率。测试结果证明了PTW的抗菌能力，表明其活性成分比可追踪化合物HNO、HNO和HO或类似物的混合组合更多。
