Department of Pharmacology, Bromatology and Toxicology, Faculty of Pharmacy and Biochemistry, Universidad Nacional Mayor de San Marcos, Jr Puno 1002, Lima 15001, Peru.
Department of Pharmacology and Therapeutics, Faculty of Veterinary Medicine, Damanhour University, Damanhour 22511, Egypt.
Molecules. 2023 Apr 10;28(8):3348. doi: 10.3390/molecules28083348.
Ruiz & Pav is known as "matico" and belongs to the Piperaceae family, and in Peru it is traditionally used as an infusion or decoction to ameliorate wound healings or ulcers. In this study, the aim was to investigate the volatile components, the antioxidant profile, and the phytotoxic activity of the essential oil (EO) of from Peru. To identify the phytoconstituents, the EO was injected into a Gas Chromatography-Mass Spectrometry (GC-MS) to obtain the chemical profile of the volatile components, followed by the antioxidant activity carried out by the reaction with three organic radicals (2,2-diphenyl-1-picrylhydrazyl (DPPH); 2,2'-azinobis-(3-ethylbenzothiazoline)-6- sulfonic acid (ABTS); ferric reducing/antioxidant power (FRAP)). Finally, the phytotoxic capabilities of the EO were tested on two model plants, seeds and bulbs. As a result, the analysis identified α-phellandrene as its main volatile chemical at 38.18%, followed by β-myrcene (29.48%) and β-phellandrene (21.88%). Regarding the antioxidant profile, the half inhibitory concentration (IC) in DPPH was 160.12 ± 0.30 µg/mL, for ABTS it was 138.10 ± 0.06 µg/mL and finally in FRAP it was 450.10 ± 0.05 µg/mL. The phytotoxic activity demonstrated that the EO had high activity at 5% and 10% against seed germination, the inhibition of root length, and hypocotyl length. Additionally, in bulbs, the inhibition root length was obtained at 10%, both comparable to glyphosate, which was used as a positive control. The molecular docking on 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) revealed that α-phellandrene had -5.8 kcal/mol, being near to glyphosate at -6.3 kcal/mol. The conclusion shows that the EO of presented antioxidant and phytotoxic activity and might be useful as a bioherbicide in the future.
Ruiz 和 Pav 被称为“matico”，属于胡椒科，在秘鲁传统上被用作煎剂或煮剂来改善伤口愈合或溃疡。在这项研究中，目的是研究来自秘鲁的 的挥发性成分、抗氧化特性和植物毒性活性。为了鉴定植物成分，将精油注入气相色谱-质谱联用仪 (GC-MS) 以获得挥发性成分的化学特征，然后通过与三种有机自由基（2,2-二苯基-1-苦基肼（DPPH）；2,2'-联氮双-（3-乙基苯并噻唑啉-6-磺酸）（ABTS）；铁还原/抗氧化能力（FRAP））的反应进行抗氧化活性分析。最后，在两种模式植物， 种子和 鳞茎上测试了精油的植物毒性能力。结果表明，分析确定 α-松油烯是其主要挥发性化学物质，含量为 38.18%，其次是 β-月桂烯（29.48%）和 β-蒎烯（21.88%）。关于抗氧化特性，DPPH 的半抑制浓度（IC）为 160.12 ± 0.30 µg/mL，ABTS 为 138.10 ± 0.06 µg/mL，FRAP 为 450.10 ± 0.05 µg/mL。植物毒性活性表明，精油在 5%和 10%浓度下对 种子萌发、根长和下胚轴长度的抑制作用具有高活性。此外，在 鳞茎中，在 10%浓度下获得了对根长的抑制作用，这与作为阳性对照的草甘膦相当。对 5-烯醇丙酮酰莽草酸-3-磷酸合酶（EPSPS）的分子对接表明，α-松油烯具有-5.8 kcal/mol，与草甘膦的-6.3 kcal/mol接近。结论表明， 的精油具有抗氧化和植物毒性活性，将来可能用作生物除草剂。
