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利用振动液柱实现大鼠心脏脱细胞自动化与优化。

Automation and Optimization of Rat Heart Decellularization Using a Vibrating Fluid Column.

机构信息

Measurements and Optical Electronics Department, Politehnica University Timisoara, 300006 Timisoara, Romania.

Chemistry and Engineering of Organic and Natural Compounds Department, University Politehnica Timisoara, 300006 Timisoara, Romania.

出版信息

Sensors (Basel). 2023 Apr 17;23(8):4045. doi: 10.3390/s23084045.

DOI:10.3390/s23084045
PMID:37112386
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10140852/
Abstract

This paper presents the validation of a software application to optimize the discoloration process in simulated hearts and to automate and determine the final moment of decellularization in rat hearts using a vibrating fluid column. The implemented algorithm specifically for the automated verification of a simulated heart's discoloration process was optimized in this study. Initially, we used a latex balloon containing enough dye to reach the opacity of a heart. The complete discoloration process corresponds to complete decellularization. The developed software automatically detects the complete discoloration of a simulated heart. Finally, the process stops automatically. Another goal was to optimize the Langendorff-type experimental apparatus, which is pressure-controlled and equipped with a vibrating fluid column that shortens the decellularization time by mechanically acting directly on cell membranes. Control experiments were performed with the designed experimental device and the vibrating liquid column using different decellularization protocols for hearts taken from rats. In this work, we used a commonly utilized solution based on sodium dodecyl sulfate. Ultraviolet spectrophotometry was used to measure the evolution of the dye concentration in the simulated hearts and, similarly, to determine the concentrations of deoxyribonucleic acid (DNA) and proteins in the rat hearts.

摘要

本文介绍了一种软件应用的验证，该软件应用可优化模拟心脏的退色过程，并使用振动液柱自动确定大鼠心脏去细胞化的最终时刻。在这项研究中，特别针对模拟心脏退色过程的自动验证实现了算法优化。最初，我们使用了一个乳胶气球，其中包含足够的染料，使其达到心脏的不透明度。完整的退色过程对应于完全去细胞化。开发的软件可自动检测模拟心脏的完全退色。最后，过程自动停止。另一个目标是优化 Langendorff 型实验设备，该设备为压力控制设备，并配备有振动液柱，通过直接作用于细胞膜，机械地缩短去细胞化时间。使用设计的实验设备和振动液柱进行了对照实验，并使用来自大鼠的心脏的不同去细胞化方案。在这项工作中，我们使用了一种常用的基于十二烷基硫酸钠的溶液。紫外分光光度法用于测量模拟心脏中染料浓度的演变，同样用于确定大鼠心脏中脱氧核糖核酸（DNA）和蛋白质的浓度。

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本文引用的文献

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Decellularized Extracellular Matrix Scaffolds for Cardiovascular Tissue Engineering: Current Techniques and Challenges.脱细胞细胞外基质支架在心血管组织工程中的应用：当前技术和挑战。
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