Thacker W L, Benson R F, Wilkinson H W, Ampel N M, Wing E J, Steigerwalt A G, Brenner D J
J Clin Microbiol. 1986 Jun;23(6):1146-7. doi: 10.1128/jcm.23.6.1146-1147.1986.
A Legionella-like organism (strain 797-PA-H; ATCC 43130) was isolated from a specimen taken from an endotracheal tube of a patient 4 days before death and from the left lung at autopsy. Growth characteristics were consistent with those for Legionella species. Strain 797-PA-H gave negative test results with available direct immunofluorescence assay conjugates and with slide agglutination test antisera prepared against the 22 Legionella species and 35 serogroups now recognized. Minimal reactivity (1 to 2 +) was observed with both tests by using reagents prepared against the Legionella-like organism Lansing 3. Reciprocal absorption studies, however, showed that the cross-reactive antibodies could be removed easily. Physiologic, gas-liquid chromatographic, and DNA hybridization tests revealed that the strain belonged to the species Legionella pneumophila. Therefore, strain 797-PA-H was designated as the type strain of a new L. pneumophila serogroup, serogroup 11.
一种类军团菌样微生物(菌株797-PA-H;ATCC 43130)是从一名患者死亡前4天取自气管内导管的标本以及尸检时取自左肺的标本中分离出来的。其生长特性与军团菌属的特性一致。菌株797-PA-H对现有的直接免疫荧光检测结合物以及针对目前已确认的22种军团菌和35个血清群制备的玻片凝集试验抗血清检测结果均为阴性。使用针对类军团菌样微生物兰辛3制备的试剂进行这两种检测时,观察到最低反应性(1至2 +)。然而,交叉吸收研究表明,交叉反应抗体很容易被去除。生理学、气液色谱和DNA杂交试验表明,该菌株属于嗜肺军团菌物种。因此,菌株797-PA-H被指定为嗜肺军团菌一个新血清群——血清群11的模式菌株。