Thacker W L, Wilkinson H W, Plikaytis B B, Steigerwalt A G, Mayberry W R, Moss C W, Brenner D J
J Clin Microbiol. 1985 Jul;22(1):1-4. doi: 10.1128/jcm.22.1.1-4.1985.
Three strains of Legionella feeleii from patients with pneumonia (425-MI-H, 691-WI-H, and 693-WI-H) and one environmental strain (713-MI-E) received at the Centers for Disease Control for reference diagnostic testing were compared with the type strain WO-44C-C3 (ATCC 35072) by DNA hybridization, chemical analysis of cellular fatty acids and ubiquinones, biochemical tests, and serological characteristics. All four isolates were assigned to the L. feeleii species on the basis of DNA hybridization results. However, strains 691-WI-H and 693-WI-H were serologically distinct from strain WO-44C-C3, as shown by their minimal reactivity (1 to 2+) with a direct immunofluorescence conjugate prepared against L. feeleii serogroup 1 (strain WO-44C-C3). Therefore, strains 691-WI-H and 693-WI-H were placed in a new L. feeleii serogroup (serogroup 2). The reference strain of L. feeleii serogroup 2 is 691-WI-H (ATCC 35849).
从肺炎患者身上分离出的三株费氏军团菌(425-MI-H、691-WI-H和693-WI-H)以及一株从环境中分离的菌株(713-MI-E),被送往疾病控制中心用于参考诊断检测。通过DNA杂交、细胞脂肪酸和泛醌的化学分析、生化试验以及血清学特征分析,将这四株菌株与模式菌株WO-44C-C3(ATCC 35072)进行了比较。根据DNA杂交结果,所有四株分离株均被归类为费氏军团菌。然而,691-WI-H和693-WI-H菌株与WO-44C-C3菌株在血清学上存在差异,这表现为它们与针对费氏军团菌血清群1(菌株WO-44C-C3)制备的直接免疫荧光结合物的反应性极低(1至2+)。因此,691-WI-H和693-WI-H菌株被归入一个新的费氏军团菌血清群(血清群2)。费氏军团菌血清群2的参考菌株为691-WI-H(ATCC 35849)。