strain ZY-312 facilitates colonic mucosa regeneration in colitis motivating STAT3 signaling pathway induced by IL-22 from ILC3 secretion.

INTRODUCTION

Probiotics play critical roles in relieving inflammatory bowel disease (IBD). However, the underlying mechanism of strain ZY-312 () for colonic mucosa regeneration in IBD remains unclear.

METHODS

The weight loss, disease activity index (DAI), colon length, and histopathology-associated index (HAI) were evaluated the therapeutic effects of in a DSS-induced colitis mouse model. Colonic mucosa proliferation and apoptosis level, and mucus density were detected by histological stain. Gut microbiota was sequenced by 16srRNA analysis. The expression of signal transducer and activator of transcription 3 (STAT3) phosphorylation in colonic mucosa was detected in -treated mice in colitis. -regulated immunity factors of motivating downstream STAT3 phosphorylation were screened by ELISA and flow cytometry. Lastly, -mediated colonic mucosa regeneration effects were verified though the knockout of STAT3 ( ) and IL-22 (IL-22) in mice, and inhibitor of STAT3 and IL-22 in co-culture model.

RESULTS

alleviated DSS-induced colitis in mice with less weight loss, DAI, colon length shortening, and HAI. Further the results showed that motivated STAT3 phosphorylation in colonic mucosa with the upregulation of proliferation index Ki-67 and mucus density, the downregulation of apoptosis level, and the modulation of gut microbiota through a mice model and STAT3 inhibitor-added model in vitro. Meanhwhile we found that promoted IL-22 production, and increased the percentage of IL-22-secreting type 3 innate lymphocytes (ILC3) in colitis. Consequently, We identified that did not increase the expression of pSTAT3, either proliferation level, mucus density, or alter gut microbiota in mice.

DISCUSSION

may indirectly motivate ILC3 to secrete IL-22, followed by IL-22-induced STAT3 phosphorylation, hence promoting colonic mucosa regeneration in colitis. It indicates that has the potential to be a biological agent for IBD therapy.