Haverkamp L J
J Neurosci. 1986 May;6(5):1338-48. doi: 10.1523/JNEUROSCI.06-05-01338.1986.
Embryos of Xenopus laevis were continually immobilized by immersion in solutions of either chloretone or lidocaine, from the late neural-fold stage to the approximate time of hatching. Such treatment has been shown to result in only transient quantitative effects on swimming behavior. Chronic immobilization was without either immediate or long-term effect on the ventral root output exhibited during "fictive" swimming episodes. Development under these conditions of diminished or absent neural activity similarly had no effects on a number of measures of the size and complexity of motoneuron dendritic arborizations. These results support the premise that the early development of specific neuronal morphology and connectivity may be largely independent of functional activity.
非洲爪蟾胚胎从神经褶晚期到接近孵化时，持续浸泡在水合氯醛或利多卡因溶液中以使其固定。已表明这种处理仅对游泳行为产生短暂的定量影响。慢性固定对“虚拟”游泳期间表现出的腹根输出既无即时影响也无长期影响。在神经活动减弱或缺失的这些条件下发育，同样对运动神经元树突分支的大小和复杂性的多项测量指标没有影响。这些结果支持这样一个前提，即特定神经元形态和连接性的早期发育可能在很大程度上独立于功能活动。
