Henan International Joint Laboratory of Animal Welfare and Health Breeding, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, China; State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, China.
State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, China; Suzhou Shangfangshan Forest Safari Park, Suzhou, China.
Poult Sci. 2023 Jun;102(6):102671. doi: 10.1016/j.psj.2023.102671. Epub 2023 Mar 24.
The search for effective in-feed antibiotic alternative is growing due to the global trend to reduce or ban the utilization of antibiotics as growth promotors in poultry diets. This study was processed to assess the effect of dietary refined functional carbohydrates (RFCs) replacing antibiotic growth promoters (AGP) on growth performance, intestinal morphologic structure and microbiota, as well as intestinal immune function and barrier function of broilers reared on a commercial broilers farm. Trials contained 3 treatments with 4 replicate broiler houses, with about 25,000 birds each room. The treatments were control group (CON), RFCs group (CON + 100 mg/kg RFCs), and AGP group (CON + 50 mg/kg bacitracin methylene disalicylate (BMD), respectively. Results showed that RFCs and AGP group significantly increased (P < 0.05) average daily gain (ADG) during d 22 to 45 in contrast to control. Compared with the control and AGP-treated groups, feeding RFCs increased (P < 0.05) jejunal villus height to crypt depth ratio. AGP addition reduced (P < 0.05) the jejunal villi surface area compared to broilers fed control and RFC supplemented diets. Supplementation of RFCs promoted (P < 0.05) the growth of Lactobacillus but inhibited Escherichia coli and Salmonella proliferation compared with the control group. Inclusion of RFCs and BMD enhanced (P < 0.05) antibody titers against avian influenza virus H9 compared with control. RFCs and AGP both down-regulated (P < 0.05) intestinal TLR4 mRNA levels, whereas RFCs tended to up-regulate (P = 0.05) IFN-γ gene expression compared to control. Expression of intestinal tight junction genes was not affected by either AGP or RFCs supplementation. Based on above observation, we suggested that RFCs could replace in-feed antibiotic BMD in broiler diets for reducing intestinal pathogenic bacteria and modulating immunity of broilers.
由于全球范围内减少或禁止在禽饲料中使用抗生素作为生长促进剂的趋势，寻找有效的饲料用抗生素替代品的需求日益增长。本研究旨在评估日粮中添加精制功能性碳水化合物（RFC）替代抗生素生长促进剂（AGP）对商业肉鸡养殖场肉鸡生长性能、肠道形态结构和微生物群、肠道免疫功能和屏障功能的影响。试验采用 3 种处理，每个处理设 4 个重复，每个重复 25000 只鸡。处理组分别为对照组（CON）、RFC 组（CON+100mg/kg RFCs）和 AGP 组（CON+50mg/kg 杆菌肽锌复合物（BMD）。结果表明，与对照组相比，RFC 组和 AGP 组在 22-45 日龄时平均日增重（ADG）显著提高（P<0.05）。与对照组和 AGP 处理组相比，添加 RFC 增加了空肠绒毛高度与隐窝深度的比值（P<0.05）。添加 AGP 降低了空肠绒毛表面积（P<0.05）与对照组和补充 RFC 日粮的肉鸡相比。与对照组相比，添加 RFC 促进了（P<0.05）乳酸杆菌的生长，但抑制了大肠杆菌和沙门氏菌的增殖。与对照组相比，添加 RFCs 和 BMD 提高了（P<0.05）针对禽流感病毒 H9 的抗体滴度。RFCs 和 AGP 均下调了（P<0.05）肠道 TLR4 mRNA 水平，而与对照组相比，RFCs 有上调（P=0.05）IFN-γ基因表达的趋势。AGP 或 RFCs 补充均不影响肠道紧密连接基因的表达。基于上述观察结果，我们认为 RFCs 可以替代肉鸡日粮中的抗生素 BMD，以减少肠道病原菌并调节肉鸡的免疫功能。
