Division of Colorectal and Anal Surgery, Department of Gastrointestinal Surgery, Guangxi Medical University Cancer Hospital, Nanning, Guangxi, China.
Department of Breast and Thyroid Surgery, The Fifth Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China.
Pathol Oncol Res. 2023 Apr 12;29:1611082. doi: 10.3389/pore.2023.1611082. eCollection 2023.
Colon cancer (CC) is the fifth most prevalent cancer around the globe and poses a major risk to human health. Even though Kremen2 serves as a prognostic indicator in individuals with malignant tumours, its role in evaluating the prognosis of individuals with colon cancer has not been confirmed. Here, we examined the protein expression of Kremen2 in CC tissues and paired adjacent normal tissues by immunohistochemistry (IHC), then analyzed the clinical and RNA-seq data presented in The Cancer Genome Atlas (TCGA) database to confirm the relationship between Kremen2 levels and CC. In addition, the associations between Kremen2 mRNA expression and infiltrating immune cells were examined. The study showed that the mRNA expression and protein level of Kremen2 were increased in CC tissues compared with adjacent normal tissues. According to Kaplan-Meier analysis, high Kremen2 expression in CC was linked to poor overall survival and progression-free survival. Clinical correlation analysis highlighted that a high level of Kremen2 expression was strongly linked with tumour progression, particularly lymph node metastasis. Cox regression analysis highlighted that Kremen2 was an independent prognostic indicator for CC. Bioinformatic studies highlighted that Kremen2 might be associated with the immune status in CC. Increased Kremen2 could serve as a potential prognostic CC biomarker.
结肠癌（CC）是全球第五大常见癌症，对人类健康构成重大威胁。尽管 Kremen2 是恶性肿瘤患者的预后指标，但它在评估结肠癌患者预后中的作用尚未得到证实。在这里，我们通过免疫组织化学（IHC）检测 CC 组织和配对的相邻正常组织中的 Kremen2 蛋白表达，然后分析美国癌症基因组图谱（TCGA）数据库中提供的临床和 RNA-seq 数据，以确认 Kremen2 水平与 CC 之间的关系。此外，还检查了 Kremen2 mRNA 表达与浸润免疫细胞之间的关联。研究表明，与相邻正常组织相比，CC 组织中 Kremen2 的 mRNA 表达和蛋白水平增加。根据 Kaplan-Meier 分析，CC 中高 Kremen2 表达与总生存期和无进展生存期不良相关。临床相关性分析强调，高水平的 Kremen2 表达与肿瘤进展，特别是淋巴结转移密切相关。Cox 回归分析强调 Kremen2 是 CC 的独立预后指标。生物信息学研究强调，Kremen2 可能与 CC 中的免疫状态有关。增加的 Kremen2 可能成为 CC 的潜在预后生物标志物。
