Sima Obiang Cédric, Ndong Mba Thiery, Ondo Joseph Privat, Ngoua Meye Misso Rick Léonid, Orango Bourdette Juliette Ornely, Otogo N'Nang Elvis, Mbogho Abogho Joefred, Jolinom Mbot Elvis, Obame Engonga Louis Clément, Nsi Emvo Edouard
Laboratoire de Recherche en Biochimie (LAREBIO), Faculté des Sciences, Université des Sciences et Techniques de Masuku, Franceville, Gabon.
Laboratoire de Substances Naturelles et de Synthèses Organométalliques (LASNSOM), Université des Sciences et Techniques de Masuku, Franceville, Gabon.
Adv Pharmacol Pharm Sci. 2023 Apr 22;2023:9304681. doi: 10.1155/2023/9304681. eCollection 2023.
Medicinal plants are traditionally used in Gabon to treat several types of illnesses. The study's purpose was to determine the toxic, antibacterial, and anti-inflammatory effects of Pierre extracts and to characterize their phytochemical compounds. Toxicity was evaluated on frog tadpoles ( Hallowell). The microorganism susceptibility test was performed by the diffusion method, while minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were evaluated using the microdilution technique. Anti-inflammatory activity was tested through protein denaturation and membrane stabilization methods. Chromatography and molecular network techniques were used to characterize chemical compounds. The lethality test showed that the lethal concentration (LC) increased from 110.03 ± 1.25 to 15.86 ± 2.21 g/mL after 24 and 96 hours of exposure. In tadpoles exposed to 7.81 g/mL extract, the first mortalities (12.5%) were observed on the fifth day of exposure. A relative decrease in mature erythrocytes exposed to plant extracts was observed. The antibacterial activity shows that the Ak F, Ak F, and Ak F fractions (from the water-ethanol crude extract) gave the greatest antibacterial activities compared to the other extracts. The water, water-acetone, and water-ethanol extracts showed good inhibition of denaturation. The haemolysis test shows that the extracts exhibited good anti-inflammatory activities. Phytochemical characterisation revealed four major compounds, including monogallate epicatechin and hydroxy-ergostadian. The molecular network revealed five main clusters. Our study shows that Pierre could be a promising natural product for the isolation of molecules with potential biological activities.
加蓬传统上使用药用植物治疗多种疾病。该研究的目的是确定皮埃尔提取物的毒性、抗菌和抗炎作用，并对其植物化学成分进行表征。对青蛙蝌蚪（哈洛威尔）进行毒性评估。采用扩散法进行微生物敏感性试验，同时使用微量稀释技术评估最低抑菌浓度（MIC）和最低杀菌浓度（MBC）。通过蛋白质变性和膜稳定化方法测试抗炎活性。采用色谱和分子网络技术对化合物进行表征。致死性试验表明，暴露24小时和96小时后，致死浓度（LC）从110.03±1.25克/毫升增加到15.86±2.21克/毫升。在暴露于7.81克/毫升提取物的蝌蚪中，在暴露的第五天观察到首次死亡（12.5%）。观察到暴露于植物提取物的成熟红细胞相对减少。抗菌活性表明，与其他提取物相比，Ak F、Ak F和Ak F馏分（来自水-乙醇粗提物）具有最大的抗菌活性。水、水-丙酮和水-乙醇提取物对变性表现出良好的抑制作用。溶血试验表明，提取物具有良好的抗炎活性。植物化学表征揭示了四种主要化合物，包括表儿茶素单没食子酸酯和羟基麦角甾烷。分子网络揭示了五个主要簇。我们的研究表明，皮埃尔可能是一种有前途的天然产物，可用于分离具有潜在生物活性的分子。
