Department of Biological Sciences, Missouri University of Science and Technology, Rolla, Missouri, USA.
Department of Material Science and Engineering, Missouri University of Science and Technology, Rolla, Missouri, USA.
J Biomed Mater Res A. 2023 Oct;111(10):1554-1564. doi: 10.1002/jbm.a.37553. Epub 2023 May 2.
Borate bioactive glasses have gained attention in recent years due to their therapeutic and regenerative effects in vivo. However, borate bioactive glasses release alkaline ions, increasing the local pH and creating a toxic environment for cell culture studies. A partial compositional substitution of phosphate for borate can create a pH-neutral glass that does not significantly affect the local pH while still releasing therapeutic ions. In the present study, a series of Na-Ca-borophosphate bioactive glasses with different borate-to-phosphate ratios was evaluated in vitro and in vivo for cytotoxicity and angiogenic effects. Compared to more basic borate glasses, the pH-neutral glasses supported endothelial cell migration and stimulated greater blood vessel formation in a chick chorioallantoic membrane model. The results from this study indicate that these pH-neutral glasses are promising angiogenic biomaterials for use in tissue engineering and regenerative medicine.
硼酸生物活性玻璃由于其在体内的治疗和再生作用近年来受到关注。然而，硼酸生物活性玻璃释放碱性离子，增加局部 pH 值，为细胞培养研究创造有毒环境。用磷酸盐部分替代硼酸盐可以创造 pH 值中性的玻璃，在不显著影响局部 pH 值的情况下仍释放治疗性离子。在本研究中，评估了一系列具有不同硼酸盐/磷酸盐比例的 Na-Ca-硼磷酸盐生物活性玻璃的体外和体内细胞毒性和血管生成作用。与碱性更强的硼酸盐玻璃相比，中性 pH 值玻璃支持内皮细胞迁移，并在鸡胚绒毛尿囊膜模型中刺激更大的血管形成。本研究结果表明，这些 pH 值中性玻璃是用于组织工程和再生医学的有前途的血管生成生物材料。
