Hageman Kara A, Blatt Rebekah L, Kuenne William A, Brow Richard K, McIff Terence E
Bioengineering Graduate Program, University of Kansas, Lawrence, KS, United States.
Department of Orthopedic Surgery and Sports Medicine, University of Kansas Medical Center, Kansas City, KS, United States.
Front Bioeng Biotechnol. 2024 Sep 18;12:1462795. doi: 10.3389/fbioe.2024.1462795. eCollection 2024.
Infection is a leading cause of total joint arthroplasty failure. Current preventative measures incorporate antibiotics into the poly (methyl methacrylate) (PMMA) bone cement that anchors the implant into the natural bone. With bacterial resistance to antibiotics on the rise, the development of alternative antibacterial materials is crucial to mitigate infection. Borate bioactive glass, 13-93-B3, has been studied previously for use in orthopedic applications due to its ability to be incorporated into bone cements and other scaffolds, convert into hydroxyapatite (HA)-like layer, and enhance the osseointegration and antibacterial properties of the material. The purpose of this study is to better understand how glass composition and change in surrounding pH effects the composite's antibacterial characteristics by comparing the incorporation of 30% wt/wt 13-93-B3 glass and pH neutral borophosphate bioactive glass into PMMA bone cement. We also aim to elucidate how HA-like layer formation on the cement's surface may affect bacterial adhesion. These studies showed that 13-93-B3 incorporated cements had significant reduction of bacterial growth surrounding the composite beyond 24 h of exposure when compared to a neutral borate bioactive glass incorporated cement ( < 0.01) and cement only ( < 0.0001). Additionally, through soaking cement composites in simulated body fluid and then exposing them to a bioluminescent strand of we found that the presence of a HA-like layer on the 13-93-B3 or pH neutral glass incorporated cement disks resulted in an increase in bacterial attachment on the composite cement's surface, where < 0.001, and < 0.05 respectively. Overall, our studies demonstrated that borate bioactive glass incorporated PMMA bone cement has innate antimicrobial properties that make it a promising material to prevent infection in total joint arthroplasties.
感染是全关节置换术失败的主要原因。目前的预防措施是将抗生素加入到聚甲基丙烯酸甲酯（PMMA）骨水泥中，这种骨水泥将植入物固定在天然骨中。随着细菌对抗生素的耐药性不断上升，开发替代抗菌材料对于减轻感染至关重要。硼酸盐生物活性玻璃13-93-B3此前已被研究用于骨科应用，因为它能够被掺入骨水泥和其他支架中，转化为类似羟基磷灰石（HA）的层，并增强材料的骨整合和抗菌性能。本研究的目的是通过比较将30%重量/重量的13-93-B3玻璃和pH中性硼磷酸盐生物活性玻璃掺入PMMA骨水泥，更好地了解玻璃成分和周围pH值的变化如何影响复合材料的抗菌特性。我们还旨在阐明水泥表面类似HA层的形成如何影响细菌粘附。这些研究表明，与掺入中性硼酸盐生物活性玻璃的水泥（<0.01）和仅水泥（<0.0001）相比，掺入13-93-B3的水泥在暴露24小时后复合材料周围的细菌生长显著减少。此外，通过将水泥复合材料浸泡在模拟体液中，然后将它们暴露于发光的菌株，我们发现13-93-B3或pH中性玻璃掺入的水泥盘上存在类似HA的层导致复合材料水泥表面的细菌附着增加，其中分别<0.001和<0.05。总体而言，我们的研究表明，掺入硼酸盐生物活性玻璃的PMMA骨水泥具有固有的抗菌特性，使其成为预防全关节置换术中感染的有前途的材料。
