From the Department of Pediatrics and Adolescent Medicine, Juntendo University Graduate School of Medicine, Tokyo, Japan.
the Department of Pediatrics Medicine, Juntendo University Faculty of Medicine, Tokyo, Japan.
J Pediatr Gastroenterol Nutr. 2023 Aug 1;77(2):e42-e47. doi: 10.1097/MPG.0000000000003805. Epub 2023 May 2.
Fetal growth restriction (FGR) indicates increased risks of lifestyle-related diseases in adulthood. Previous studies showed the association between human gut dysbiosis and various diseases. However, reports examining the relationship between FGR and gut microbiota are scarce. Herein, we hypothesized that FGR may cause gut dysbiosis and analyzed the gut microbiota in a FGR rat model by restricting maternal protein intake during pregnancy.
The FGR group was developed by feeding pregnant Sprague Dawley rats a diet containing 7% protein until birth. Control rats were fed 21% protein. Fecal samples of 2-11-week-old pups were collected weekly. DNA was extracted from each sample and subjected to polymerase chain reaction (PCR) amplification and sequencing. Additionally, short-chain fatty acids in the cecum were analyzed at 2 weeks of age, when there were differences in the occupancy of the gut microbiota.
Comparative analysis of the gut microbiota showed differences only at 2 weeks of age. Verrucomicrobia was significantly more abundant in the control group ( q < 0.1), whereas pathogenic bacteria, including Enterococcus and Enterobacteriaceae , tended to increase in the FGR group. The abundance of acetic and butyric acid-producing bacteria also differed between groups. Acetic acid in the cecum was considerably decreased in the FGR group, while butyric acid was increased compared to that in the control group.
Normalizing the alteration of FGR on postnatal gut microbiota may have beneficial effects for the host, since the FGR group caused gut dysbiosis.
胎儿生长受限（FGR）表明成年后患与生活方式相关疾病的风险增加。先前的研究表明，人类肠道菌群失调与各种疾病之间存在关联。然而，关于 FGR 与肠道微生物群之间关系的报告很少。在此，我们假设 FGR 可能导致肠道菌群失调，并通过在妊娠期间限制母体蛋白质摄入来分析 FGR 大鼠模型中的肠道微生物群。
FGR 组通过在妊娠期间给怀孕的 Sprague Dawley 大鼠喂食含有 7%蛋白质的饮食直至分娩来建立。对照组大鼠喂食 21%蛋白质。每周收集 2-11 周龄幼鼠的粪便样本。从每个样本中提取 DNA，并进行聚合酶链反应（PCR）扩增和测序。此外，在 2 周龄时，当肠道微生物群的定植存在差异时，分析盲肠中的短链脂肪酸。
肠道微生物群的比较分析仅在 2 周龄时显示出差异。对照组中厚壁菌门明显更丰富（q < 0.1），而致病性细菌，包括肠球菌和肠杆菌科，在 FGR 组中趋于增加。产乙酸和丁酸细菌的丰度也在两组之间存在差异。FGR 组盲肠中的乙酸显著减少，而丁酸与对照组相比增加。
正常化 FGR 对出生后肠道微生物群的改变可能对宿主有益，因为 FGR 组导致肠道菌群失调。
