Mayberry-Carson K J, Mayberry W R, Tober-Meyer B K, Costerton J W, Lambe D W
Microbios. 1986;45(182):21-32.
Discs of rabbit tibia, 5 mm thick, were utilized to study the adherence of Staphylococcus aureus to the bone surface in the presence and absence of clindamycin. Bacteria were grown in broth media containing the bone slices and varying concentrations of clindamycin. In the absence of the antibiotic, S. aureus adhered extensively to bone surfaces and formed large microcolonies which were surrounded by an amorphous matrix. In the presence of 0.025 micrograms/ml of clindamycin (0.1 MIC), S. aureus adhered less to bone surfaces, forming smaller and fewer microcolonies. In the presence of 0.0625 micrograms/ml of clindamycin (0.25 MIC), S. aureus adhered to the bone surfaces only sparsely, forming small microcolonies with very little matrix holding them together, and leaving very large areas of the bone surface uncolonized. In the presence of 0.125 micrograms/ml of clindamycin (0.5 MIC), bone surfaces were basically clean, with only one or two cells (no microcolonies) found in crevices and indentations of the bone surface. In the presence of 0.25 micrograms/ml (1 MIC) no bacteria adhered to the bone surfaces.
采用5毫米厚的兔胫骨椎间盘来研究在有或没有克林霉素存在的情况下金黄色葡萄球菌对骨表面的黏附情况。细菌在含有骨切片和不同浓度克林霉素的肉汤培养基中生长。在没有抗生素的情况下，金黄色葡萄球菌广泛黏附于骨表面并形成大的微菌落，这些微菌落被无定形基质包围。在含有0.025微克/毫升克林霉素（0.1倍最低抑菌浓度）的情况下，金黄色葡萄球菌对骨表面的黏附较少，形成的微菌落更小且数量更少。在含有0.0625微克/毫升克林霉素（0.25倍最低抑菌浓度）的情况下，金黄色葡萄球菌仅稀疏地黏附于骨表面，形成小的微菌落，将它们聚集在一起的基质很少，骨表面有很大区域未被定植。在含有0.125微克/毫升克林霉素（0.5倍最低抑菌浓度）的情况下，骨表面基本干净，仅在骨表面的缝隙和凹陷处发现一两个细胞（没有微菌落）。在含有0.25微克/毫升（1倍最低抑菌浓度）的情况下，没有细菌黏附于骨表面。
