Evans R P, Nelson C L, Bowen W R, Kleve M G, Hickmon S G
Cherry Creek Orthopedic Surgery, Denver, CO 80209, USA.
Clin Orthop Relat Res. 1998 Feb(347):243-9.
Using a new technique for scanning electron microscopic preparation, bacterial glycocalyx is seen in its natural, highly hydrated state for the first time. Visual images of the glycocalyx obtained from these preparations are a marked departure from the visual images of glycocalyx obtained previously with conventional scanning electron microscopic analysis. The dominating presence of glycocalyx visualized in its naturally hydrated state gives credence to the role of bacterial glycocalyx as a mechanical barrier to host defenses and antibiotics and supports the role of glycocalyx as a significant factor in bacterial virulence.
使用一种扫描电子显微镜制备的新技术，首次观察到处于天然高水合状态的细菌糖萼。从这些制备物中获得的糖萼视觉图像与先前通过传统扫描电子显微镜分析获得的糖萼视觉图像有显著差异。在其天然水合状态下可视化的糖萼的主要存在证实了细菌糖萼作为宿主防御和抗生素的机械屏障的作用，并支持糖萼作为细菌毒力的重要因素的作用。
