Center of Excellence in Natural Products for Ageing and Chronic Diseases, Chulalongkorn University, Bangkok 10330, Thailand; Pharmaceutical Sciences and Technology Program, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
Center of Excellence in Natural Products for Ageing and Chronic Diseases, Chulalongkorn University, Bangkok 10330, Thailand; Department of Pharmacology and Physiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
Int J Biol Macromol. 2023 Jul 1;242(Pt 1):124673. doi: 10.1016/j.ijbiomac.2023.124673. Epub 2023 May 1.
Magnetic drug targeting can be a strategy for effectively delivering phytochemicals in cancer treatment. Here, we demonstrate the benefit of magnetic targeting with superparamagnetic iron oxide nanoparticles for cytotoxicity enhancement of lutein (LUT) against breast cancer cells. Fabrication of LUT-loaded chitosan/alginate iron oxide nanoparticles (LUT-CS/Alg-FeO-NPs) was optimized by a statistical approach using response surface methodology based on the Box-Behnken design. The optimized LUT-CS/Alg-FeO-NPs with a balance among LUT concentration, copolymer coating, and iron ion concentration exhibited controlled size, narrow size distribution, better crystallinity, excellent saturation magnetization, and sustained-release profile. The negligible magnetic coercivity and remanent magnetization confirmed the superparamagnetism of the prepared NPs. The optimized LUT-CS/Alg-FeO-NPs were biocompatible while exhibiting a significantly enhanced cytotoxicity towards breast cancer MCF-7 cells upon exposure to a permanent magnet compared to free LUT with a 4-fold increase, suggesting the potential of LUT-CS/Alg-FeO-NPs as magnetically targeted delivery for breast cancer.
磁靶向药物可以成为癌症治疗中有效递送植物化学物质的一种策略。在这里，我们通过超顺磁性氧化铁纳米粒子的磁靶向作用，展示了叶黄素（LUT）对乳腺癌细胞的细胞毒性增强作用的益处。通过基于 Box-Behnken 设计的响应面法的统计方法，优化了载有 LUT 的壳聚糖/海藻酸钠氧化铁纳米粒子（LUT-CS/Alg-FeO-NPs）的制备。优化后的 LUT-CS/Alg-FeO-NPs 在 LUT 浓度、共聚物涂层和铁离子浓度之间达到平衡，表现出可控的尺寸、窄的尺寸分布、更好的结晶度、优异的饱和磁化强度和持续释放特性。可以忽略的磁矫顽力和剩余磁化强度证实了所制备的 NPs 的超顺磁性。优化后的 LUT-CS/Alg-FeO-NPs 具有生物相容性，在暴露于永磁体时对乳腺癌 MCF-7 细胞表现出显著增强的细胞毒性，与游离 LUT 相比增加了 4 倍，这表明 LUT-CS/Alg-FeO-NPs 作为乳腺癌的磁性靶向递送具有潜力。
