海参毒素和卡泊芬净对诱导后的菌株阴道细胞炎症参数的影响。

The Effects of Holothurin and Caspofungin on the Vaginal Cell Inflammation Parameters of the Strain Post Induction of .

作者信息

Handayani Sri, Nurdiana Nurdiana, Winarsih Sri, Tri Endharti Agustina

机构信息

Doctoral Program of Medical Science Department, Faculty of Medicine, Brawijaya University, Malang, Indonesia.

Department of Midwifery of Aisyiyah, Institution of Surakarta University, Surakarta, Province of Middle Java, Indonesia.

出版信息

Infect Drug Resist. 2023 Apr 27;16:2501-2509. doi: 10.2147/IDR.S400314. eCollection 2023.

DOI:10.2147/IDR.S400314

PMID:37138836

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10150739/

Abstract

PURPOSE

() is a fungus that causes superficial and invasive candidiasis in its host. Caspofungin, has been widely used as a synthetic antifungal, whereas holothurin has been shown to have potential as a natural antifungal. The purpose of this study was to see how holothurin and caspofungin affected the number of colonies, LDH levels, and the number of inflammatory cells in vagina of .

PATIENTS AND METHODS

Design of this research is using posttest only with control group design with 48 Wistar strains used in this study were divided into six treatment groups. Each group was divided into three-time intervals of 12, 24, and 48 hours. LDH markers were tested using ELISA, inflammatory cells were counted manually, and the number of colonies was calculated using colonymetry before being diluted with NaCl 0.9% and planted in sabouraud dextrose agar (SDA).

RESULTS

According to the findings, inflammatory cells in the treatment of holothurin (48-hours) had an OR of 1.68 CI (-0.79-4.16) P = 0.09 and caspofungin had an OR of 4.18 CI (1.26-9.63) P = 0.09. Meanwhile, LDH in the holothurin (48-hour) treatment obtained OR 348, CI (286-410), P=0.03, and Caspofungin OR 393, CI (277-508), P=0.03. Colonies were obtained with zero numbers in the holothurin treatment (48 hours) and with Caspofungin OR 393, CI (273-508) P=0.00.

CONCLUSION

Holothurin and caspofungin administration reduced the number of colonies and the number of inflammatory cells (P 0.05), implying that holothurin and caspofungin could prevent infection.

摘要

目的

（）是一种可在宿主体内引起浅表性和侵袭性念珠菌病的真菌。卡泊芬净作为一种合成抗真菌药物已被广泛使用，而海参毒素已显示出具有作为天然抗真菌剂的潜力。本研究的目的是观察海参毒素和卡泊芬净如何影响（）阴道中的菌落数量、乳酸脱氢酶（LDH）水平以及炎症细胞数量。

患者与方法

本研究采用仅后测的对照组设计，本研究中使用的48只Wistar品系动物被分为六个治疗组。每组又分为12小时、24小时和48小时三个时间间隔。使用酶联免疫吸附测定法（ELISA）检测LDH标志物，手动计数炎症细胞，在先用0.9%氯化钠稀释并接种于沙氏葡萄糖琼脂（SDA）之前，使用菌落计数法计算菌落数量。

结果

根据研究结果，海参毒素治疗组（48小时）的炎症细胞比值比（OR）为1.68，置信区间（CI）为（-0.79 - 4.16），P = 0.09；卡泊芬净治疗组的OR为4.18，CI为（1.26 - 9.63），P = 0.09。同时，海参毒素治疗组（48小时）的LDH的OR为348，CI为（286 - 410），P = 0.03；卡泊芬净治疗组的OR为393，CI为（277 - 508），P = 0.03。海参毒素治疗组（48小时）的菌落数量为零，卡泊芬净治疗组的OR为393，CI为（273 - 508），P = 0.00。