Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, Orlando, FL, USA.
Methods Mol Biol. 2023;2668:159-189. doi: 10.1007/978-1-0716-3203-1_13.
Extracellular vesicles (EVs) transport nucleic acids, proteins, and lipid molecules for intercellular communication. The biomolecular cargo from EVs can modify the recipient cell genetically, physiologically, and pathologically. This innate ability of EVs can be harnessed to deliver the cargo of interest to a specific organ or a cell type. Importantly, due to their ability to cross the blood-brain barrier (BBB), the EVs can be used as delivery vehicles to transport therapeutic drugs and other macromolecules to inaccessible organs such as the brain. Therefore, the current chapter includes laboratory techniques and protocols focusing on the customization of EVs for neuronal research.
细胞外囊泡 (EVs) 可运输核酸、蛋白质和脂类分子，从而实现细胞间通讯。EVs 中的生物分子货物可以遗传、生理和病理上改变受体细胞。EVs 的这种固有能力可用于将感兴趣的货物递送到特定器官或细胞类型。重要的是，由于它们能够穿过血脑屏障 (BBB)，因此 EVs 可用作递药载体，将治疗性药物和其他大分子递送到大脑等难以到达的器官。因此，本章包括专注于 EVs 定制用于神经元研究的实验室技术和方案。
