Laboratory of Developmental Biology, Disease Networks Research Unit, Faculty of Biochemistry and Molecular Medicine, University of Oulu and Kvantum Institute, Oulu, Finland.
Department of Chemistry, National Taiwan University, Taipei, Taiwan.
Methods Mol Biol. 2023;2668:241-256. doi: 10.1007/978-1-0716-3203-1_17.
Integrating the versatility of synthetic nanoparticles to natural biomaterials, such as cells or cell membranes, has gained considerable attention as promising alternative cargo delivery platforms in recent years. Extracellular vesicles (EVs), natural nanomaterials composed of a protein-rich lipid bilayer secreted by cells, have also shown advantages and great potential as a nano delivery platform in combination with synthetic particles due to their specific natural properties in overcoming several biology hurdles possessed in the recipient cell. Therefore, the preservation of EV's origin properties is critical for their application as nanocarriers. This chapter will describe the encapsulation procedure of MSN encapsulated in EV membrane derived from mouse renal adenocarcinoma (Renca) cells through biogenesis. The FMSN-enclosed EVs produced through this approach still contain preserved EV's natural membrane properties.
近年来，将合成纳米粒子的多功能性与细胞或细胞膜等天然生物材料相结合，作为有前途的货物输送平台引起了相当大的关注。细胞分泌的富含蛋白质的脂质双层组成的细胞外囊泡 (EVs) 作为一种天然纳米材料，也因其在克服受体细胞中存在的几种生物学障碍方面的特定天然特性，作为纳米输送平台与合成颗粒结合显示出优势和巨大潜力。因此，作为纳米载体，EV 起源特性的保留对于其应用至关重要。本章将描述通过生物发生将 MSN 封装在源自小鼠肾腺癌 (Renca) 细胞的 EV 膜中的封装程序。通过这种方法产生的 FMSN 封闭的 EV 仍然包含保留的 EV 的天然膜特性。
