Ortín J, Nájera R, López C, Dávila M, Domingo E
Gene. 1980 Nov;11(3-4):319-31. doi: 10.1016/0378-1119(80)90072-4.
The genetic heterogeneity of five influenza A (H3N2) strains isolated between 1968 and 1977 has been estimated by T1-oligonucleotide fingerprinting of 32P-labeled viral RNA. Assuming that the large T1-resistant oligonucleotides represent a random sample of the viral RNA, the genetic differences observed would affect 0.3 to 10.7% of the RNA positions of the genes studied, depending on the pair of viruses considered. A smaller degree of genetic heterogeneity was observed when six coetaneous viral samples were compared. The distribution of spontaneous mutations among the viral genes was studied by fingerprinting individual RNA segments isolated either by gel electrophoresis or hybridization with plasmids containing influenza-specific DNA sequences. No statistically significant differences were detected in the distribution of mutations among the viral genes studied. The mutation frequency at the hemagglutinin RNA region coding for the HA1 subunit was found to be two times higher than that at the region encoding that HA2 subunit. Our results suggest that the antigenic variability of influenza viruses may be a consequence of a general genetic variability which effects many of the viral genes.
通过对32P标记的病毒RNA进行T1 - 寡核苷酸指纹图谱分析，估算了1968年至1977年间分离出的五株甲型流感（H3N2）病毒株的遗传异质性。假设大的T1抗性寡核苷酸代表病毒RNA的随机样本，观察到的遗传差异会影响所研究基因RNA位置的0.3%至10.7%，具体取决于所考虑的病毒对。比较六个同期病毒样本时，观察到的遗传异质性程度较小。通过对通过凝胶电泳或与含有流感特异性DNA序列的质粒杂交分离出的单个RNA片段进行指纹图谱分析，研究了病毒基因中自发突变的分布。在所研究的病毒基因中，未检测到突变分布的统计学显著差异。发现编码HA1亚基的血凝素RNA区域的突变频率比编码HA2亚基的区域高两倍。我们的结果表明，流感病毒的抗原变异性可能是影响许多病毒基因的一般遗传变异性的结果。
