Bevan M, Barker R, Goldsbrough A, Jarvis M, Kavanagh T, Iturriaga G
Nucleic Acids Res. 1986 Jun 11;14(11):4625-38. doi: 10.1093/nar/14.11.4625.
We have isolated recombinant lambda clones containing intact major tuber protein (patatin) genes and flanking sequences from the commercial tetraploid variety Maris Piper. The gene is composed of seven exons and six introns, spread over 4 kb of DNA. Nuclease mapping defined the 5' end of the mRNA approximately 45 bp upstream of the initiation codon. The 5' end of the gene is preceeded by a canonical TATA box sequence. The three known patatin genes encode proteins of nearly identical Mr but very different isoelectric points. The sequence of the gene does not indicate a role for patatin as one of the globulin class of plant storage proteins.
我们从商业四倍体品种马里斯·派珀（Maris Piper）中分离出了含有完整主要块茎蛋白（马铃薯素）基因及侧翼序列的重组λ克隆。该基因由7个外显子和6个内含子组成，分布在4kb的DNA上。核酸酶图谱分析确定了mRNA的5'端位于起始密码子上游约45bp处。该基因的5'端之前有一个典型的TATA盒序列。三个已知的马铃薯素基因编码的蛋白质分子量几乎相同，但等电点差异很大。该基因的序列并未表明马铃薯素在植物贮藏蛋白球蛋白类中发挥作用。
