Blundy K S, Blundy M A, Carter D, Wilson F, Park W D, Burrell M M
Advanced Technologies (Cambridge) Ltd., England.
Plant Mol Biol. 1991 Jan;16(1):153-60. doi: 10.1007/BF00017925.
Patatin is a family of glycoproteins that contributes about 40% of the total soluble protein in tubers of potato (Solanum tuberosum L.). The protein is encoded by a multigene family of 50-70 genes which have been divided into classes I and II on the basis of sequence homology. The promoters of two class I genes, PS20 and PS3/27, were transcriptionally fused to beta-glucuronidase and transformed into the potato cultivars Désirée and Maris Bard. Examination of the expression levels in large populations of microtubers indicated that the PS20 promoter produced beta-glucuronidase activities 5-fold lower in Désirée than Maris Bard whereas the PS3/27 promoter showed similar levels in both cultivars. Furthermore, the relative expression levels from the two promoters were reversed in the two cultivars. The beta-glucuronidase enzyme activity was correlated with the mRNA level but not the copy number of the introduced gene. The implications for the use of patatin promoters in the genetic modification of tubers is discussed.
马铃薯Patatin是一类糖蛋白，约占马铃薯（Solanum tuberosum L.）块茎中总可溶性蛋白的40%。该蛋白由一个包含50 - 70个基因的多基因家族编码，根据序列同源性已被分为I类和II类。将两个I类基因PS20和PS3/27的启动子与β-葡萄糖醛酸酶进行转录融合，并转化到马铃薯品种Désirée和Maris Bard中。对大量微型块茎中的表达水平进行检测表明，PS20启动子在Désirée中产生的β-葡萄糖醛酸酶活性比Maris Bard低5倍，而PS3/27启动子在两个品种中的表达水平相似。此外，两个启动子的相对表达水平在两个品种中相反。β-葡萄糖醛酸酶活性与mRNA水平相关，但与导入基因的拷贝数无关。文中讨论了Patatin启动子在块茎基因改造中的应用意义。
