AFRICA CMM Medical Mycology Research Unit, Institute of Infectious Disease and Molecular Medicine (IDM), Cape Town, South Africa.
Department of Pathology, Faculty of Health Sciences, University of Cape Town, Cape Town, South Africa.
Methods Mol Biol. 2023;2667:169-179. doi: 10.1007/978-1-0716-3199-7_13.
Pneumocystis jirovecii causes pneumonia in immunocompromised patients. A major challenge in drug susceptibility testing and in understanding host/pathogen interactions is that Pneumocystis spp. are not viable in vitro. Continuous culture of the organism is not currently available, and therefore, developing new drug targets is very limited. Due to this limitation, mouse models of Pneumocystis pneumonia have proven to be an invaluable resource to researchers. In this chapter, we provide an overview of selected methods used in mouse models of infection including, in vivo Pneumocystis murina propagation, routes of transmission, genetic mouse models available, a P. murina life form-specific model, a mouse model of PCP immune reconstitution inflammatory syndrome (IRIS), and the experimental parameters associated with these models.
肺孢子菌引起免疫功能低下患者的肺炎。药物敏感性测试和了解宿主/病原体相互作用的主要挑战是，肺孢子菌在体外不可存活。该生物的连续培养目前不可用，因此，新的药物靶点的开发非常有限。由于这一限制，肺孢子菌肺炎的小鼠模型已被证明是研究人员非常宝贵的资源。在本章中，我们提供了感染小鼠模型中使用的选定方法的概述，包括体内传播、传播途径、现有的遗传小鼠模型、肺孢子菌生活形态特异性模型、PCP 免疫重建炎症综合征 (IRIS) 的小鼠模型，以及与这些模型相关的实验参数。
