Department of Food Science and Technology, Nutrition and Food Security Research Center, School of Nutrition and Food Science, Isfahan University of Medical Sciences, Isfahan, Iran.
Infectious Diseases and Tropical Medicine Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
Probiotics Antimicrob Proteins. 2024 Jun;16(3):784-795. doi: 10.1007/s12602-023-10081-7. Epub 2023 May 5.
The effectiveness of selenium nanoparticle (SeNP)-enriched Lactiplantibacillus plantarum and Lactobacillus acidophilus was studied against Shiga toxin-producing Escherichia coli O157:H7 infection on the intestinal fragments and kidney tissue of BALB/c mice. Gut microbiota-targeted bacteria and E. coli O157:H7 counts were obtained by qPCR and PCR. Histology of ileum, colon, and kidney tissues and Stx secretions were analyzed until one-week post-infection. Mice fed with SeNP Lpb. plantarum in the preinfection feeding groups have lower E. coli O157:H7 counts and lower intestinal damage than those in the infected group. The lowest mean fecal probiotic counts were in the L. acidophilus group (7.61 log 10). In pretreatment groups of SeNP L. acidophilus and L. acidophilus, the mean counts of bacteria decreased to 10 CFU/g by day 7. The lowest Stx copy number was demonstrated in SeNP Lpb. plantarum feeding groups' day 7 (P < 0.05). Feeding groups with SeNP Lpb. plantarum had significantly higher members of Lactobacilli in their fecal microbiota than the control group on day 7. It was clarified that Se-enriched Lpb. plantarum and L. acidophilus can be useful as a method of preventing STEC infections. The viability of STEC infection exposure to selenium-enriched Lactobacillus spp. was decreased more than for non-Se-enriched Lactobacillus spp.
富硒纳米颗粒（SeNP）强化植物乳杆菌和嗜酸乳杆菌对肠道片段和 BALB/c 小鼠肾脏组织中产志贺毒素大肠杆菌 O157:H7 感染的有效性进行了研究。通过 qPCR 和 PCR 获得了靶向肠道微生物群的细菌和大肠杆菌 O157:H7 的计数。分析了回肠、结肠和肾脏组织的组织学以及 Stx 的分泌情况，直到感染后一周。在感染前喂养组中用 SeNP Lpb. plantarum 喂养的小鼠的大肠杆菌 O157:H7 计数和肠道损伤低于感染组。嗜酸乳杆菌组的平均益生菌计数最低（7.61 log 10）。在 SeNP 嗜酸乳杆菌和嗜酸乳杆菌的预处理组中，细菌数量在第 7 天减少到 10 CFU/g。在 SeNP Lpb. plantarum 喂养组的第 7 天，Stx 拷贝数最低（P < 0.05）。在第 7 天，与对照组相比，用 SeNP Lpb. plantarum 喂养的组的粪便微生物群中乳杆菌的成员显著增加。结果表明，富硒植物乳杆菌和嗜酸乳杆菌可作为预防 STEC 感染的方法。暴露于富硒乳杆菌的 STEC 感染的活力比非富硒乳杆菌降低更多。
