采用定量实时聚合酶链反应对功能性便秘患儿肠道菌群进行定量分析
Quantification of Intestinal Species in Children with Functional Constipation by Quantitative Real-Time PCR.
作者信息
Jomehzadeh Nabi, Javaherizadeh Hazhir, Amin Mansour, Rashno Mohammad, Teimoori Ali
机构信息
Cellular and Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
出版信息
Clin Exp Gastroenterol. 2020 May 5;13:141-150. doi: 10.2147/CEG.S250755. eCollection 2020.
BACKGROUND
Functional constipation is a common and annoying gastrointestinal disorder among children worldwide in which the intestinal microbiota composition plays a fundamental role. This study aimed to compare the quantity of main intestinal species in constipated children and healthy controls.
MATERIALS AND METHODS
Fecal samples were collected from 40 children fulfilling Rome IV criteria for functional constipation and 40 healthy volunteers. Quantitative real-time polymerase chain reaction (qPCR) method with species-specific primers was used to investigate seven main species in fecal samples.
RESULTS
strains of the patient group were different from the healthy controls, main differences being noticeable decrease in the population quantity of (mean 10 CFU/gram feces) and lower abundance of (p<0.0001).
CONCLUSION
species might be quantitatively altered in childhood functional constipation. Our findings on the different species of population showed significantly decreased quantity in the patient group compared with the healthy subjects.
背景
功能性便秘是一种在全球儿童中常见且令人烦恼的胃肠道疾病，其中肠道微生物群组成起着重要作用。本研究旨在比较便秘儿童和健康对照中主要肠道菌种的数量。
材料与方法
从40名符合罗马IV功能性便秘标准的儿童和40名健康志愿者中收集粪便样本。使用带有物种特异性引物的定量实时聚合酶链反应（qPCR）方法研究粪便样本中的七种主要菌种。
结果
患者组的菌株与健康对照不同，主要差异在于（平均每克粪便10 CFU）的种群数量显著减少以及（p<0.0001）的丰度较低。
结论
在儿童功能性便秘中，菌种数量可能会发生改变。我们关于不同菌种数量的研究结果显示，与健康受试者相比，患者组的数量显著减少。
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