Ninghai Institute of Mariculture Breeding and Seed Industry, Zhejiang Wanli University, Ningbo, 315000, China.
Zhejiang Key Laboratory of Aquatic Germplasm Resource, College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo, 315000, China.
Curr Microbiol. 2023 May 5;80(6):203. doi: 10.1007/s00284-023-03310-3.
With the rapid development of intensive aquaculture, the considerable release of nitrogenous organic compounds has become a serious threat to aquatic organisms. Currently, isolating autochthonous aerobic denitrifying bacteria (ADB) from aquaculture environments is essential for the biological elimination of nitrogenous pollutants. In this study, the enrichment of ADB from shrimp pond water and sediment samples was conducted under different shaking durations. The absolute abundance of total bacteria, nosZ-type, and the napA-type ADB was measured using qPCR. High-throughput sequencing of 16S rRNA, nosZ, and napA genes was performed to reveal the community structure of bacteria and ADB, respectively. Our data revealed that absolute abundance and the community structure of the total bacteria, nosZ-type and napA-type ADB, were significantly altered under different shaking durations. Specifically, the order Pseudomonadales, possessing both nosZ and napA genes, was significantly enriched in water and sediment samples under both 12/12 and 24/0 shaking/static cycles. However, in water samples, compared to the 24/0 shaking/static cycles, the 12/12 shaking/static cycles could lead to a higher enrichment rate of aerobic denitrification bacteria indicated by the higher absolute abundance of bacteria and the higher accounting percentage of orders Oceanospirillales and Vibrionales. Moreover, although the order Pseudomonadales notably increased under the 12/12 of shake/static cycle compared to the 24/0 shaking/static cycle, considering the relative higher abundance of ADB in 24/0 shaking/static cycle, the enrichment of ADB in sediment may be efficient with the 24/0 shaking/static cycle.
随着集约化水产养殖的快速发展，相当数量的含氮有机化合物的排放对水生生物构成了严重威胁。目前，从水产养殖环境中分离土著好氧反硝化细菌（ADB）对于生物去除含氮污染物至关重要。在这项研究中，通过不同的摇床时间对虾塘水和底泥样品中的 ADB 进行了富集。使用 qPCR 测量了总细菌、nosZ 型和 napA 型 ADB 的绝对丰度。分别对 16S rRNA、nosZ 和 napA 基因进行高通量测序，以揭示细菌和 ADB 的群落结构。我们的数据表明，在不同的摇床时间下，总细菌、nosZ 型和 napA 型 ADB 的绝对丰度和群落结构都发生了显著变化。具体而言，具有 nosZ 和 napA 基因的假单胞菌目在水和底泥样品中均在 12/12 和 24/0 摇床/静置循环下显著富集。然而，在水样中，与 24/0 摇床/静置循环相比，12/12 摇床/静置循环可导致好氧反硝化细菌的绝对丰度更高，细菌和海洋螺旋菌目和颤蚓目占比更高，表明其富集率更高。此外，尽管与 24/0 摇床/静置循环相比，12/12 摇床/静置循环下假单胞菌目明显增加，但考虑到 24/0 摇床/静置循环中 ADB 的相对丰度较高，24/0 摇床/静置循环可能更有利于底泥中 ADB 的富集。
