茯苓及其提取物对脾气虚证大鼠补脾作用的影响。
Effectiveness of Fuling () and its extracts against spleen deficiency in rats tonifying spleen.
机构信息
Hubei Provincial Key Laboratory of Applied Toxicology, Hubei Provincial Center for Disease Control and Prevention, Wuhan 430075, China.
Department of pharmaceutical affairs, Hubei Provincial Hospital of TCM, Wuhan 430074, China.
出版信息
J Tradit Chin Med. 2023 Jun;43(3):501-506. doi: 10.19852/j.cnki.jtcm.2023.03.002.
OBJECTIVE
To observe and explore the effect of Fuling () in alleviating the spleen deficiency symptom pattern (SDSP).
METHODS
We established an animal model of SDS in Sprague-Dawley () rats by treating them with deficiency-inducing factors, including irregular feeding and tail clamping. Mice were administered Fuling () and its extracts (raw/cooked powder, aqueous/alcohol extract) by gavage once a day for 21 d. The body weight, rectal temperature, and spleen and thymus organ coefficients were calculated. The levels of motilin (MTL), gastrin (GAS), aquaporin 2 (AQP2), interleukin 2 (IL-2), IL-4, and 5-hydroxytryptamine (5-HT) in the serum and the level of AQP2 in the kidneys were evaluated by enzyme-linked immunosorbent assay.
RESULTS
Fuling () and its extracts did not change the body weight, rectal temperature, and organ coefficients of the spleen and thymus. However, it reduced the levels of MTL and GAS and increased the levels of IL-2 and AQP2. In addition, the levels of IL-4 and 5-HT showed no significant alteration.
CONCLUSIONS
These results suggested the crucial function of () in SDSP, especially promoting digestive function and water metabolism.
目的
观察并探讨茯苓（）缓解脾虚证（SDSP）的作用。
方法
采用不规则饮食和夹尾等致虚因素复制 SDSP 大鼠模型，SDSP 小鼠给予茯苓及其提取物（生/熟粉、水/醇提物）灌胃，每日 1 次，连续 21 天。计算体质量、直肠温度、脾脏和胸腺脏器系数。采用酶联免疫吸附试验检测血清胃动素（MTL）、胃泌素（GAS）、水通道蛋白 2（AQP2）、白细胞介素 2（IL-2）、IL-4 和 5-羟色胺（5-HT）水平及肾脏 AQP2 水平。
结果
茯苓及其提取物不影响体质量、直肠温度和脾脏、胸腺脏器系数，但可降低 MTL 和 GAS 水平，升高 IL-2 和 AQP2 水平，IL-4 和 5-HT 水平无明显变化。
结论
茯苓在 SDSP 中发挥重要作用，尤其可促进消化功能和水液代谢。
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