电针阳陵泉可增加脑血流，改善手术线栓法致大脑中动脉闭塞模型大鼠的痉挛性瘫痪状态。
Waggle needling Yanglingquan (GB34) enhances cerebral blood flow and ameliorates condition of spastic paresis in rats with middle cerebral artery occlusion induced by surgical nylon suture.
机构信息
School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China.
2 the Key Unit of State Administration of Traditional Chines Medicine, Evaluation of Characteristic Acupuncture Therapy, Beijing 100029, China.
出版信息
J Tradit Chin Med. 2023 Jun;43(3):514-522. doi: 10.19852/j.cnki.jtcm.20230328.001.
OBJECTIVE
To evaluate the therapeutic efficacy and the influence on cerebral blood supply of waggle needling Yanglingquan (GB34) on spastic paresis (SP) rats after middle cerebral artery occlusion (MCAO) induced and investigate its mechanism of relieving neurobehavior deficiency.
METHODS
SP rat model was produced by permanent MCAO. Rats were divided into five groups: blank control group (Control), sham operation group (Sham), model group (Model), waggle needling group (WN) and perpendicular needling group (PN). SP rats were treated with acupuncture from day 3 after MCAO, once a day for 6 d. The modified neurological severity score (mNSS) and modified Ashworth scale (MAS) were conducted on days 0, 1, 3, 5, 7 and 9. Cerebral blood flow (CBF) in ischemic cortex was measured by laser speckle imaging 5 min pre ischemia, 5 min post ischemia, and after intervention on day 9. All rats were sacrificed at day 9 and the protein and mRNA expressions of γ2 subunit of the γ-aminobutyric acid receptor A (GABAAγ2) and K+-Cl?cotransporter 2 (KCC2) in the ischemic cortex and lumber enlargement was measured by Western blotting and real-time quantitative polymerase chain reaction.
RESULTS
Both Control and Sham groups showed no changes in mNSS and MAS scores and in the regional CBF. Compared with Model group, both WN and PN treatments significantly ameliorated neurological deficit ( 0.01), decreased muscle tone ( 0.05), and enhanced CBF ( 0.001) in SP rats; moreover, WN showed superior effects than PN ( 0.001). In line with the improvement in neurobehavior, acupuncture interventions up-regulated the expressions of GABAAγ2 and KCC2 in the ischemic cortex as well as lumber enlargement ( 0.01) in SP rats, and those changes were more obvious in WN ( 0.05).
CONCLUSIONS
Acupuncture at Yanglingquan (GB34) enhanced cerebral blood flow and ameliorated SP in permanent MCAO rats, while waggle needling was superior to regular perpendicular needling. Waggle needling Yanglingquan (GB34) would be a potential complementary therapy for SP.
目的
评价摆动针法针刺阳陵泉（GB34）对大脑中动脉闭塞（MCAO）后痉挛性瘫痪（SP）大鼠的治疗效果及对脑血流的影响，并探讨其缓解神经功能缺损的机制。
方法
采用永久性 MCAO 制作 SP 大鼠模型。将大鼠分为空白对照组（Control）、假手术组（Sham）、模型组（Model）、摆动针法组（WN）和直刺针法组（PN）。MCAO 后 3 天开始对 SP 大鼠进行针刺治疗，每天 1 次，共 6 天。在 0、1、3、5、7 和 9 天进行改良神经功能缺损评分（mNSS）和改良 Ashworth 量表（MAS）评分。在缺血皮质内用激光散斑成像测量缺血前 5 分钟、缺血后 5 分钟和第 9 天干预后的脑血流（CBF）。第 9 天处死所有大鼠，采用 Western blot 和实时定量聚合酶链反应法测量缺血皮质和脊髓增粗区 γ2 亚基 γ-氨基丁酸 A 型受体（GABAAγ2）和 K+-Cl?共转运蛋白 2（KCC2）的蛋白和 mRNA 表达。
结果
空白对照组和假手术组的 mNSS 和 MAS 评分及局部 CBF 均无变化。与模型组相比，WN 和 PN 治疗均显著改善 SP 大鼠的神经功能缺损（ 0.01），降低肌肉张力（ 0.05），并增强 CBF（ 0.001）；此外，WN 治疗效果优于 PN（ 0.001）。与神经行为改善一致，针刺干预上调了 SP 大鼠缺血皮质及脊髓增粗区 GABAAγ2 和 KCC2 的表达（ 0.01），WN 组的变化更为明显（ 0.05）。
结论
针刺阳陵泉（GB34）可增强大脑中动脉闭塞永久性 MCAO 大鼠的脑血流，改善 SP，摆动针法优于常规直刺针法。摆动针法针刺阳陵泉（GB34）可能是 SP 的一种潜在的补充治疗方法。
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