Sultana Nikhath, Ahmed Shafath, Nandini Vidyashree V, Lathief Jailance, Boruah Shiney
Prosthodontics, SRM (Sri Ramaswamy Memorial) Kattankulathur Dental College and Hospital, Chennai, IND.
Cureus. 2023 Apr 3;15(4):e37085. doi: 10.7759/cureus.37085. eCollection 2023 Apr.
The purpose of this in vitro study is to compare and evaluate the surface roughness and microbial adhesion of and after the finishing and polishing of three different denture base materials.
A total of 84 samples of three different denture materials were used. The samples were divided into three groups: Group I (conventional poly methyl methacrylate), Group II (injection-molded polymethyl methacrylate), and Group III (injection-molded polyamide). Fourteen samples from each group were tested for surface roughness using an optical profilometer. Seven samples from each group were incubated in a suitable culture broth containing and separately for 48 hours. Microbial colony forming unit (cfu/ml) was estimated in order to evaluate the microbial adhesion to the surface of the denture base materials. Confocal laser scanning microscopy was done to visualize the microorganisms.
The mean surface roughness of Group I was 0.1176± 0.04 µm, Group II was 0.0669±0.02 µm, Group III was 0.1971±0.02 µm. One-way ANOVA revealed statistically significant differences in the mean surface roughness values among the three groups (p < 0.05). Tukey HSD (honestly significant difference) test confirmed the specific differences within the groups. The results of colony forming unit showed maximum adherence in Group III samples among both the species followed by Group I samples and least in Group II samples. Confocal laser scanning microscopy revealed significant differences in microbial adhesion among both and in the three groups (p <0.05). One-way multivariate ANOVA was performed to analyze the data obtained from confocal laser scanning microscopy. Microbial adhesion was least observed in Group II samples followed by Group I samples and the highest microbial adhesion was observed in Group III samples.
Microbial adhesion was proved to have a direct correlation with the surface roughness of denture base materials. An increase in surface roughness (Ra) increases microbial adhesion.
本体外研究的目的是比较和评估三种不同义齿基托材料完成和抛光后的表面粗糙度及微生物黏附情况。
共使用了三种不同义齿材料的84个样本。样本分为三组：第一组（传统聚甲基丙烯酸甲酯）、第二组（注塑聚甲基丙烯酸甲酯）和第三组（注塑聚酰胺）。每组14个样本使用光学轮廓仪测试表面粗糙度。每组7个样本分别在含有两种细菌的合适培养液中孵育48小时。估计微生物菌落形成单位（cfu/ml）以评估微生物对义齿基托材料表面的黏附情况。进行共聚焦激光扫描显微镜检查以观察微生物。
第一组的平均表面粗糙度为0.1176±0.04 µm，第二组为0.0669±0.02 µm，第三组为0.1971±0.02 µm。单因素方差分析显示三组之间的平均表面粗糙度值存在统计学显著差异（p<0.05）。Tukey HSD（真实显著差异）检验证实了组内的具体差异。菌落形成单位的结果显示，在两种细菌中，第三组样本的黏附性最强，其次是第一组样本，第二组样本的黏附性最低。共聚焦激光扫描显微镜显示三组中两种细菌的微生物黏附存在显著差异（p<0.05）。进行单因素多变量方差分析以分析从共聚焦激光扫描显微镜获得的数据。在第二组样本中观察到的微生物黏附最少随后是第一组样本，在第三组样本中观察到的微生物黏附最高。
证明微生物黏附与义齿基托材料的表面粗糙度直接相关。表面粗糙度（Ra）增加会增加微生物黏附。
