Mayahara Mitsuori, Kataoka Ryuta, Arimoto Takafumi, Tamaki Yukimichi, Yamaguchi Nobuaki, Watanabe Yuki, Yamasaki Yoshizumi, Miyazaki Takashi
Department of Special Needs Dentistry, Division of Dental Education, School of Dentistry, Showa University, Tokyo, Japan.
J Investig Clin Dent. 2014 Nov;5(4):307-12. doi: 10.1111/jicd.12055. Epub 2013 Jun 14.
Candida albicans is a common oral fungus but can cause serious conditions such as Candida stomatitis. We investigated C. albicans adhesion to the surface of denture-base resins at two growth phases.
Fungal suspensions of logarithmic (9 h) and stationary phase (24 h) C. albicans (JCM2085) were used. Scanning electron microscopy (SEM) confirmed that yeast and mycelial forms were predominant in 9-h and 24-h cultures, respectively. Resin strips were polished to three surface roughness levels (Ra 3.2 μm, Ra 0.48 μm and Ra 0.06 μm) and were then immersed in C. albicans suspensions for both phases. The SEM images were taken at five sites on each strip.
Adhesion of mycelial-form C. albicans on rough surfaces (Ra = 3.2) was 2.2 times higher than on smooth surfaces (Ra = 0.06; 7030 vs 3580 adhesions/mm(2), P < 0.01). The hyphae of these mycelial forms fully penetrated the surface cracks. Fewer adhesions occurred for yeast-form C. albicans, regardless of surface type (440-620 adhesions/mm(2), P = n.s.).
Adhesion of yeast-form C. albicans was indifferent to surface roughness. In contrast, mycelial adhesion increased with surface roughness of the resin because mycelia infiltrated the minute protuberances on rough surfaces.
白色念珠菌是一种常见的口腔真菌，但可引发如念珠菌性口炎等严重病症。我们研究了白色念珠菌在两个生长阶段对义齿基托树脂表面的黏附情况。
使用对数期（9小时）和稳定期（24小时）的白色念珠菌（JCM2085）真菌悬液。扫描电子显微镜（SEM）证实，在9小时和24小时培养物中，酵母形式和菌丝形式分别占主导。将树脂条抛光至三种表面粗糙度水平（Ra 3.2μm、Ra 0.48μm和Ra 0.06μm），然后将其浸入两个阶段的白色念珠菌悬液中。在每条树脂条的五个部位拍摄SEM图像。
菌丝形式的白色念珠菌在粗糙表面（Ra = 3.2）上的黏附力比在光滑表面（Ra = 0.06）上高2.2倍（分别为7030个/mm²和3580个/mm²，P < 0.01）。这些菌丝形式的菌丝完全穿透了表面裂缝。无论表面类型如何，酵母形式的白色念珠菌黏附较少（440 - 620个/mm²，P值无统计学意义）。
酵母形式的白色念珠菌黏附对表面粗糙度不敏感。相比之下，菌丝黏附随着树脂表面粗糙度的增加而增加，因为菌丝会渗入粗糙表面的微小凸起中。
