Moon Hyungkyu, Sultana Tania, Lee JeongIk, Huh Jungrim, Lee Hae Dong, Choi Myung-Seok
Department of Materials Chemistry and Engineering, Konkuk University, Seoul, Republic of Korea.
Regenerative Medicine Laboratory, Center for Stem Cell Research, Department of Biomedical Science and Technology, Institute of Biomedical Science and Technology, Konkuk University, Seoul, Republic of Korea.
Front Chem. 2023 Apr 21;11:1151526. doi: 10.3389/fchem.2023.1151526. eCollection 2023.
Fluorescence probe is one of the most powerful tools for cellular imaging. Here, three phospholipid-mimicking fluorescent probes (FP1-FP3) comprising fluorescein and two lipophilic groups of saturated and/or unsaturated C18 fatty acids were synthesized, and their optical properties were investigated. Like in biological phospholipids, the fluorescein group acts as a hydrophilic polar headgroup and the lipid groups act as hydrophobic non-polar tail groups. Laser confocal microscope images illustrated that FP3, which contains both saturated and unsaturated lipid tails, showed great uptake into the canine adipose-derived mesenchymal stem cells.
荧光探针是细胞成像最强大的工具之一。在此，合成了三种由荧光素和两个饱和和/或不饱和C18脂肪酸的亲脂性基团组成的模拟磷脂荧光探针（FP1-FP3），并研究了它们的光学性质。如同在生物磷脂中一样，荧光素基团充当亲水性极性头部基团，脂质基团充当疏水性非极性尾部基团。激光共聚焦显微镜图像表明，含有饱和和不饱和脂质尾部的FP3对犬脂肪来源的间充质干细胞具有很强的摄取能力。
