Karaoğlu Öznur, Serhatlı Müge, Pelvan Ebru, Karadeniz Bülent, Demirtas Ilknur, Çakırca Gamze, Sipahix Hande, Özhan Yağmur, Karapınar Gözdem, Charehsaz Mohammad, Aydın Ahmet, Yesilada Erdem, Alasalvar Cesarettin
Life Sciences, TÜBİTAK Marmara Research Center, Gebze-Kocaeli, Turkey.
Department of Molecular Biology and Genetics, Faculty of Science, Gebze Technical University, Gebze-Kocaeli, Turkey.
J Funct Foods. 2023 Jun;105:105544. doi: 10.1016/j.jff.2023.105544. Epub 2023 Apr 19.
Prevention of COVID-19 is of paramount importance for public health. Some natural extracts might have the potential to suppress COVID-19 infection. Therefore, this study aimed to design a standardised, efficient, and safe chewable tablet formulation (with propolis and three herbal extracts) for possible prevention against two variants (Wuhan B.1.36 and Omicron BA.1.1) of SARS-CoV-2 virus and other viral infections. Green tea, bilberry, dried pomegranate peel, and propolis extracts were selected for this purpose. Cytotoxicity and antiviral activity of each component, as well as the developed chewable tablet, were examined against severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) virus using Vero E6 cells with the xCELLigence real-time cell analyser-multiple plates system. Anti-inflammatory and analgesic activities, as well as mutagenicity and anti-mutagenicity of the chewable tablet were also analysed. Compared to the control, it was observed that the chewable tablet at concentrations of 110 and 55 µg/mL had antiviral activity rates of 101% and 81%, respectively, for the Wuhan variant and 112% and 35%, respectively, for the Omicron variant. The combination of herbal extracts with propolis extract were synergically more effective (∼7-fold higher) than that of individual extract. The present work suggests that a combination of herbal extracts with propolis at suitable concentrations can effectively be used as a food supplement for the prevention of both variants of the SARS-CoV-2 virus in the oral cavity (the first entry point of the SARS-CoV-2 virus).
预防新冠病毒对公共卫生至关重要。一些天然提取物可能具有抑制新冠病毒感染的潜力。因此，本研究旨在设计一种标准化、高效且安全的咀嚼片配方（含蜂胶和三种草药提取物），用于预防严重急性呼吸综合征冠状病毒2（SARS-CoV-2）病毒的两种变体（武汉B.1.36和奥密克戎BA.1.1）以及其他病毒感染。为此选择了绿茶、越橘、干石榴皮和蜂胶提取物。使用xCELLigence实时细胞分析仪-多板系统，在Vero E6细胞上检测了每种成分以及所研制咀嚼片对严重急性呼吸综合征冠状病毒2（SARS-CoV-2）病毒的细胞毒性和抗病毒活性。还分析了咀嚼片的抗炎和镇痛活性以及致突变性和抗突变性。与对照组相比，观察到浓度为110和55μg/mL的咀嚼片对武汉变体的抗病毒活性率分别为101%和81%，对奥密克戎变体的抗病毒活性率分别为112%和35%。草药提取物与蜂胶提取物的组合协同作用效果更佳（约高7倍）。目前的研究表明，适当浓度的草药提取物与蜂胶的组合可有效用作食品补充剂，用于预防口腔（SARS-CoV-2病毒的首个进入点）中的两种SARS-CoV-2病毒变体。
