Jiangxi Provincial People's Hospital & The First Affiliated Hospital of Nanchang Medical College, Nanchang, China.
Beijing University of Chinese Medicine, Beijing, China.
Epilepsia Open. 2023 Sep;8(3):912-917. doi: 10.1002/epi4.12758. Epub 2023 May 15.
Observational studies have shown an association between 25-hydroxyvitamin D (25 (OH) D) and epilepsy, but it is unclear whether the association is causal. Therefore, we applied Mendelian randomization (MR) analysis to determine the causal relationship between serum 25 (OH) D levels and epilepsy.
We conducted a two-sample Mendelian randomization (TSMR) study to investigate the association between serum 25 (OH) D levels and epilepsy using pooled statistics from genome-wide association studies (GWAS). Data for 25 (OH) D came from a GWAS comprising 417,580 participants, and data for epilepsy were obtained from the International League Against Epilepsy (ILAE) consortium. Five methods were used to analyze TSMR, including the inverse variance weighting method, MR Egger method, weighted median method, simple model, and weighted model. In the sensitivity analysis, MR Egger and MR PRESSO methods were used to test for pleiotropy, inverse variance weighting and MR Egger in Cochran's Q statistics were used to test for heterogeneity.
MR analyzed the relationship between 25 (OH) D and different types of epilepsy, and the results showed that a 1 standard deviation increase in natural log-transformed serum 25 (OH) D levels was associated with reduced risk for juvenile absence epilepsy (IVW OR = 0.985; 95% confidence interval [CI]: 0.971-0.999; P-value = 0.038). There was no apparent heterogeneity and horizontal gene pleiotropy.
Higher serum levels of 25 (OH) D were a protective factor for adolescent absence epilepsy, but had no effect on other types of epilepsy.
观察性研究表明 25-羟维生素 D（25（OH）D）与癫痫之间存在关联，但尚不清楚这种关联是否具有因果关系。因此，我们应用孟德尔随机化（MR）分析来确定血清 25（OH）D 水平与癫痫之间的因果关系。
我们进行了两样本孟德尔随机化（TSMR）研究，使用来自全基因组关联研究（GWAS）的汇总统计数据来调查血清 25（OH）D 水平与癫痫之间的关联。25（OH）D 数据来自包含 417580 名参与者的 GWAS，癫痫数据来自国际抗癫痫联盟（ILAE）联盟。使用五种方法进行 TSMR 分析，包括逆方差加权法、MR Egger 法、加权中位数法、简单模型和加权模型。在敏感性分析中，使用 MR Egger 和 MR PRESSO 方法来检验偏倚，使用 Cochran's Q 统计量中的逆方差加权和 MR Egger 来检验异质性。
MR 分析了 25（OH）D 与不同类型癫痫之间的关系，结果表明，自然对数转换后血清 25（OH）D 水平每增加 1 个标准差，青少年失神性癫痫的风险降低（IVW OR=0.985；95%置信区间[CI]：0.971-0.999；P 值=0.038）。没有明显的异质性和水平基因偏倚。
较高的血清 25（OH）D 水平是青少年失神性癫痫的保护因素，但对其他类型的癫痫没有影响。
