Afyon Kocatepe University, Faculty of Science and Arts, Department of Chemistry, 03200 Afyonkarahisar, Türkiye.
Afyon Kocatepe University, Faculty of Science and Arts, Department of Chemistry, 03200 Afyonkarahisar, Türkiye; Samarkand State University, Faculty of Chemistry, Department of Organic and Bioorganic Chemistry, University bvld-15, Samarkand, Uzbekistan.
Int J Biol Macromol. 2023 Jul 1;242(Pt 1):124753. doi: 10.1016/j.ijbiomac.2023.124753. Epub 2023 May 7.
In this study, poly(2-(3,5-dichloroanilino)-2-oxoethyl 2-methylprop-2-enoate) (PDCOEMA), a new synthetic polymer based on methacrylate, was synthesized and characterized. The blend of PDCOEMA with chitosan (CS) was prepared by the hydrothermal method and the DSC technique confirmed its formation. It was observed that PDCOEMA increased the thermal stability and glass transition temperature (Tg) of CS. The Tg value of the PDCOEMA-CS blend was increased at about 7 °C with the highest ZnO NPs contribution rate. PDCOEMA-CS/ZnO nanocomposites were prepared by adding ZnO NPs produced by biosynthesis at different weight ratios to the PDCOEMA-CS blend by hydrothermal method. When the thermal stability of nanocomposites determined by TGA was examined, it was observed that it increased significantly compared to CS. While the initial decomposition temperature of CS was 270 °C, this value increased to 293 °C after blending with DCOEMA, and to 317 °C with the addition of 7 % ZnO NPs. Antimicrobial, anticancer, cytotoxic, antioxidant, and wound healing properties of PDCOEMA, CS, PDCOEMA-CS blend, and nanocomposites were determined. According to the obtained results, it was observed that nanocomposites containing 5 % and 7 % ZnO NPs showed good anticancer activity against A549 cells at a dose of 10 μg/mL. The results show that the produced nanocomposites can contribute to developing CS-based materials.
在这项研究中，合成了一种基于甲基丙烯酸酯的新型聚合物聚(2-(3,5-二氯苯胺基)-2-氧代乙基 2-甲基丙-2-烯酸酯)（PDCOEMA），并对其进行了表征。通过水热法制备了 PDCOEMA 与壳聚糖（CS）的共混物，并用差示扫描量热法（DSC）确认了其形成。结果表明，PDCOEMA 提高了 CS 的热稳定性和玻璃化转变温度（Tg）。PDCOEMA-CS 共混物的 Tg 值在最高 ZnO NPs 贡献率时约升高了 7°C。通过水热法将生物合成制备的 ZnO NPs 以不同的重量比添加到 PDCOEMA-CS 共混物中，制备了 PDCOEMA-CS/ZnO 纳米复合材料。当通过 TGA 测定纳米复合材料的热稳定性时，与 CS 相比，其显著增加。当 CS 的初始分解温度为 270°C 时，与 DCOEMA 共混后，该值增加到 293°C，添加 7% ZnO NPs 后增加到 317°C。测定了 PDCOEMA、CS、PDCOEMA-CS 共混物和纳米复合材料的抗菌、抗癌、细胞毒性、抗氧化和伤口愈合性能。根据获得的结果，观察到含有 5%和 7% ZnO NPs 的纳米复合材料在 10μg/mL 剂量下对 A549 细胞表现出良好的抗癌活性。结果表明，所制备的纳米复合材料可以为开发基于 CS 的材料做出贡献。
