Yang Feng, Ruixin Yang, Xiaochun Ma, Fan Zhang, Junbin Liu, Pengmei Dong, Guoyan Jiang
College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, China.
Exp Dermatol. 2023 Sep;32(9):1361-1370. doi: 10.1111/exd.14831. Epub 2023 May 9.
It has become increasingly clear that gut microbiota and skin are interconnected since the discovery of the 'gut-brain-skin' axis. Hair follicles (HFs) are skin microorganisms, but few studies have investigated their relationship to gut microbiota. Hence, we hypothesize that HFs have a close relationship with the gut, similarly to what was reported for the skin. Using rex rabbits as an animal model, one hundred healthy half-sibling rex rabbits were selected for the experiment, and 16 s rRNA gene sequencing was performed on the cecal microbiota of nine rabbits with the extremely high (HS) and low (LS) hair density (n = 9 per group) to determine differences between the composition and function of these communities. In comparison with the LS group, several alpha diversity index values were significantly lower in the HS group, although the higher variation in species composition in the HS group. Additionally, species diversity and abundance differed significantly in the cecum microbiota of HS and LS rabbits. Further, primary and secondary HF density was significantly correlated with the families Muribaculaceae and Bacteroidaceae, and genera Blautia, Bacteroides and Desulfovibrio. In particular, Muribaculaceae, Bacteroidaceae, Blautia and Bacteroides may support the development of HFs. Moreover, the expression of WNT4, WNT10a, WNT10b, CTNNB1 (β-catenin) and LEF1 in the skin was significantly higher in the HS group compared with the LS group. Altogether, the results of this study suggest that the extremely high density of HF in rabbits is associated with a significantly different microbiota diversity and community structure, and the Wnt/β-catenin signalling pathway was activated in the HS group. Thus, key bacteria may promote the development of HF.
自从发现“肠-脑-皮肤”轴以来，肠道微生物群与皮肤之间的联系日益明显。毛囊是皮肤微生物，但很少有研究调查它们与肠道微生物群的关系。因此，我们假设毛囊与肠道关系密切，类似于已报道的毛囊与皮肤的关系。本研究以獭兔作为动物模型，选取100只健康的半同胞獭兔进行实验，对9只毛发密度极高（HS）和极低（LS）的兔子（每组n = 9）的盲肠微生物群进行16 s rRNA基因测序，以确定这些群落的组成和功能差异。与LS组相比，HS组的几个α多样性指数值显著更低，尽管HS组的物种组成变化更大。此外，HS和LS兔子的盲肠微生物群的物种多样性和丰度存在显著差异。此外，初级和次级毛囊密度与毛螺菌科、拟杆菌科以及布劳特氏菌属、拟杆菌属和脱硫弧菌属显著相关。特别是，毛螺菌科、拟杆菌科、布劳特氏菌属和拟杆菌属可能支持毛囊的发育。此外，与LS组相比，HS组皮肤中WNT4、WNT10a、WNT10b、CTNNB1（β-连环蛋白）和LEF1的表达显著更高。总之，本研究结果表明，兔子中极高的毛囊密度与显著不同的微生物群多样性和群落结构相关，并且HS组中Wnt/β-连环蛋白信号通路被激活。因此，关键细菌可能促进毛囊的发育。
