Department of Preventive Dentistry, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and Vrije Universiteit Amsterdam, Amsterdam, The Netherlands.
Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and Vrije Universiteit Amsterdam, Amsterdam, The Netherlands.
Crit Rev Microbiol. 2024 Aug;50(4):397-416. doi: 10.1080/1040841X.2023.2211665. Epub 2023 May 11.
The oral cavity is inhabited by abundant microbes which continuously interact with the host and influence the host's health. Such host-microbe interactions (HMI) are dynamic and complex processes involving e.g. oral tissues, microbial communities and saliva. Due to difficulties in mimicking the complexity, it is still unclear how exactly HMI influence the transition between healthy status and disease conditions in the oral cavity. As an advanced approach, three-dimensional (3D) organotypic oral tissues (epithelium and mucosa/gingiva) are being increasingly used to study underlying mechanisms. These models were designed with different complexity depending on the research questions to be answered. In this review, we summarised the existing 3D oral HMI models, comparing designs and readouts, discussing applications as well as future perspectives.
口腔中栖息着丰富的微生物，它们与宿主不断相互作用，影响宿主的健康。这种宿主-微生物相互作用（HMI）是一个动态而复杂的过程，涉及口腔组织、微生物群落和唾液等。由于难以模拟其复杂性，目前仍不清楚 HMI 究竟如何影响口腔中健康状态与疾病状态之间的转变。作为一种先进的方法，三维（3D）器官型口腔组织（上皮和黏膜/牙龈）越来越多地被用于研究潜在机制。这些模型的设计具有不同的复杂性，具体取决于要回答的研究问题。在这篇综述中，我们总结了现有的 3D 口腔 HMI 模型，比较了设计和检测结果，讨论了它们的应用以及未来展望。
