植物中的无标记定量蛋白质组学

Label-Free Quantitative Proteomics in Plant.

作者信息

Wang Ruonan, Zhou Peijun, Pan Yilin, Zheng Lu, Dong Xiaoying, Shen Renfang, Lan Ping

机构信息

State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, China.

University of Chinese Academy of Sciences, Beijing, China.

出版信息

Methods Mol Biol. 2023;2665:75-83. doi: 10.1007/978-1-0716-3183-6_7.

DOI:10.1007/978-1-0716-3183-6_7

PMID:37166594

Abstract

Label-free quantitation (LFQ) proteomics, mainly based on the extraction of the peptide (precursor) intensity at the MS (mass spectrum 1) level, enables to quantify the relative amount of the proteins among samples. In an LFQ proteomics study, all samples are scanned individually on an advanced mass spectrometer and the chromatographic features of each run are extracted to generate consensus patterns among various runs in the experiment. Here, we describe the LFQ proteomics experimental protocol adapted for plant research, such as plant iron homeostasis.

摘要

无标记定量（LFQ）蛋白质组学主要基于在质谱（质谱1）水平提取肽（前体）强度，能够对样品间蛋白质的相对含量进行定量。在一项LFQ蛋白质组学研究中，所有样品在先进的质谱仪上单独扫描，并提取每次运行的色谱特征，以在实验的不同运行之间生成一致模式。在此，我们描述了适用于植物研究（如植物铁稳态）的LFQ蛋白质组学实验方案。