Glaesener Anne G, Merchant Sabeeha S
California Institute for Quantitative Biosciences (QB3), University of California, Berkeley, CA, USA.
Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA.
Methods Mol Biol. 2023;2665:203-215. doi: 10.1007/978-1-0716-3183-6_16.
Chlamydomonas is an excellent reference system for dissecting the impact of iron (Fe) nutrition on photosynthetic and other metabolisms. The operational definition of four stages of Fe nutrition is described and a guide to the practical use of these stages is offered, specifically the preparation of media and growth of mixotrophic cultures. A key consideration is the impact of carbon metabolism on the expression of Fe-containing enzymes and hence the Fe quota. The absolute concentration of Fe in the medium is less determinative of gene expression than the Fe available on a per-cell basis. In nature, algal cells may transition from Fe-replete to -deficient to -limited during a bloom.
衣藻是剖析铁（Fe）营养对光合作用及其他代谢影响的优良参考体系。文中描述了铁营养四个阶段的操作定义，并给出了这些阶段实际应用的指南，特别是培养基的制备和混合营养培养物的生长。一个关键的考虑因素是碳代谢对含铁酶表达以及铁配额的影响。培养基中铁的绝对浓度对基因表达的决定性作用小于基于每个细胞可得的铁。在自然界中，藻类细胞在水华期间可能会从铁充足状态转变为铁缺乏再到铁受限状态。
